Jan 27, 2004

Absence of microdeletions in the azoospermia-factor region of the Y-chromosome in Viennese men seeking assisted reproduction

Wiener klinische Wochenschrift
Christian J GruberJohannes C Huber

Abstract

The azoospermia-factor region of the Y-chromosome is essential for spermatogenesis in humans. In the literature, a wide range is given for the frequency of microdeletions in this region. The purpose of this study was to evaluate our own population of patients. During a two-year period at Vienna Medical School, all male patients (n = 383) seeking assisted reproduction were screened for microdeletions. Thirty-three men had azoospermia and 154 severe oligozoospermia. Genomic DNA was prepared from peripheral lymphocytes and polymerase chain reaction analysis of the azoospermia-factor region was performed using the Promega kit. No case tested positive for azoospermia-factor microdeletions. In all cases amplification of 18 non-polymorphic sequence tagged sites was obtained. Y-chromosome microdeletions do not seem to be an important factor for male infertility in our patients. This suggests that screening should be restricted to men with azoospermia or severe oligozoospermia only.

  • References13
  • Citations3

Citations

Mentioned in this Paper

Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Sequence Tagged Sites
Genetic Screening Method
Promega
Polymerase Chain Reaction Analysis
Chromosomes, Human, Y
Gene Amplification Technique
Partial Monosomy
Assisted Reproductive Technologies
Spermatogenesis

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