Absence of Mycobacterium avium ss paratuberculosis-specific IS900 sequence in intestinal biopsy tissues of Indian patients with Crohn's disease.

Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology
Mitnala SasikalaD Bhaskara Reddy

Abstract

The role of Mycobacterium avium ss paratuberculosis (MAP) in the etiopathology of Crohn's disease (CD) remains controversial, because of conflicting reports demonstrating the presence of MAP-specific insertion sequence from intestinal biopsy tissues of patients clinically diagnosed for the disease. The present study was carried out to investigate the presence of MAP DNA in the intestinal tissues of CD patients to ascertain the relevance of MAP in Indian patients with CD. Patients diagnosed as CD at our institute were recruited. Healthy individuals without inflammatory bowel disease served as controls. Mucosal biopsy specimens were collected from ileum and colon in duplicates and subjected to histopathological examination and polymerase chain reaction (PCR) amplification. Total DNA (81 CD patients, 85 healthy individuals) and total RNA (12 CD patients, 12 healthy individuals) isolated from tissue specimens was used for amplification of MAP-specific IS900 by nested PCR. MAP-specific IS900 DNA and RNA could not be detected by nested PCR in the intestinal tissues of any patient with CD. Our results do not support the etiological role of MAP in the pathogenesis of CD in Indian patients.

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Citations

Jan 29, 2010·Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology·Govind K Makharia, Urvashi B Singh
Jan 17, 2012·Critical Reviews in Microbiology·Rodrick J ChiodiniRichard W McCallum
May 28, 2014·International Journal of Medical Microbiology : IJMM·Pittu Sandhya RaniNiyaz Ahmed
Apr 11, 2012·Veterinary Immunology and Immunopathology·Eiichi MomotaniMasahiro Ikegami
Jun 28, 2014·Veterinary Microbiology·James W WynneWojtek P Michalski
Apr 1, 2017·The Indian Journal of Medical Research·Ajay Vir SinghVirendra Singh Yadav

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