Absence of p300 induces cellular phenotypic changes characteristic of epithelial to mesenchyme transition.

British Journal of Cancer
D KrubasikG Murphy

Abstract

p300 is a transcriptional cofactor and prototype histone acetyltransferase involved in regulating multiple cellular processes. We generated p300 deficient (p300-) cells from the colon carcinoma cell line HCT116 by gene targeting. Comparison of epithelial and mesenchymal proteins in p300- with parental HCT116 cells showed that a number of genes involved in cell and extracellular matrix interactions, typical of 'epithelial to mesenchyme transition' were differentially regulated at both the RNA and protein level. p300- cells were found to have aggressive 'cancer' phenotypes, with loss of cell-cell adhesion, defects in cell-matrix adhesion and increased migration through collagen and matrigel. Although migration was shown to be metalloproteinase mediated, these cells actually showed a downregulation or no change in the level of key metalloproteinases, indicating that changes in cellular adhesion properties can be critical for cellular mobility.

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Citations

Mar 17, 2009·Nature Cell Biology·J Bradley ZucheroR Dyche Mullins
Mar 20, 2009·The Journal of Biological Chemistry·Erin A Kimbrel, Andrew L Kung
Sep 9, 2011·PLoS Pathogens·Heather L HowieDenise A Galloway
Dec 7, 2007·FEBS Letters·Longchuan Bai, Juanita L Merchant
Feb 19, 2013·The Journal of Membrane Biology·Vladimir AjdžanovićIvan Spasojević
Feb 19, 2016·Biotechnology and Bioengineering·Aleksander SkardalShay Soker
Oct 22, 2013·Journal of Surgical Oncology·Jung Wook HuhHo-Kyung Chun
Dec 21, 2019·World Journal of Surgical Oncology·Felix RühlmannSebastian Dango
Apr 21, 2011·Journal of Cellular Physiology·Sanja MijatovicFerdinando Nicoletti
Apr 14, 2016·Journal of Cellular and Molecular Medicine·Darina L Lazarova, Michael Bordonaro
Mar 28, 2017·Breast Cancer Research and Treatment·Muhammad AsaduzzamanErnesto Yagüe

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Datasets Mentioned

BETA
GM6001

Methods Mentioned

BETA
acetylation
FCS
flow cytometry

Related Concepts

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