Abstract
To determine if peptides containing the 'shared epitope' sequence, QKRAA, from either endogenous, HLA-DR beta 1 (0401), or exogenous, Escherichia coli dnaJ, sources activate T cells in recent onset rheumatoid arthritis (RA). Peripheral blood mononuclear cell (PBMC) proliferative and whole blood cytokine responses to shared epitope containing peptides from DR beta 1 (0401) and E coli dnaJ, to control peptides from DR beta 1 (0402) and hsp40 and to the recall antigen, tetanus toxoid, were tested in 20 untreated, recent onset RA subjects, 20 HLA, age, and sex matched healthy controls and 18 other subjects with inflammatory arthritis. PBMC proliferative responses to a second E coli dnaJ peptide (with the shared epitope at the N-terminus) and two peptides from type II collagen with high affinity for DR4(0401) were tested in a further 16 recent onset RA and 17 control subjects. PBMC proliferation and whole blood interferon gamma or interleukin 10 production in response to the shared epitope containing and control peptides were not different between the disease and control groups. On the other hand, compared with controls, RA subjects had significantly higher proliferation to a collagen II (aa 1307-1319) peptide, but significantly low...Continue Reading
References
Nov 1, 1992·Cytokine·P F ZangerleP Franchimont
Jun 1, 1992·Clinical Biochemistry·S Albani, J Roudier
Jan 1, 1991·Autoimmunity·E KeystoneM Harth
Mar 1, 1990·Journal of Clinical Immunology·J VerwilghenJ L Ceuppens
Oct 1, 1986·Arthritis and Rheumatism·G C TsokosA H Rook
Nov 1, 1987·Arthritis and Rheumatism·P K GregersenR J Winchester
Jan 1, 1989·Springer Seminars in Immunopathology·P E LipskyN Oppenheimer-Marks
Jan 1, 1989·Arthritis and Rheumatism·G T NepomB S Nepom
Feb 1, 1987·Arthritis and Rheumatism·M G Malaise, P Franchimont
Jan 1, 1988·Scandinavian Journal of Immunology·T StolzenburgF J Wagenhaeuser
Jan 1, 1988·Rheumatology International·M SeitzH Kirchner
Mar 1, 1988·Arthritis and Rheumatism·F C ArnettH S Luthra
Jul 1, 1988·The Journal of Pathology·A J Freemont
Dec 1, 1987·Annals of the Rheumatic Diseases·A J Freemont
Oct 1, 1984·The Journal of Clinical Investigation·D G MaloneR L Wilder
Sep 1, 1983·Arthritis and Rheumatism·S M WahlJ L Decker
Jan 1, 1995·The Journal of Experimental Medicine·M SalmiS Jalkanen
Oct 12, 1995·Journal of Immunological Methods·N Petrovsky, L C Harrison
May 1, 1995·Nature Medicine·S AlbaniD A Carson
Jul 1, 1995·Arthritis and Rheumatism·S B CohenM Feldmann
May 1, 1995·The Journal of Experimental Medicine·J HammerF Sinigaglia
Jul 1, 1994·The Journal of Clinical Investigation·M K CrowK B Elkon
Aug 1, 1994·The Journal of Clinical Investigation·A P CopeM Feldmann
Oct 1, 1993·British Journal of Rheumatology·A J SilmanW E Ollier
Dec 1, 1995·Seminars in Immunology·A Y Rudensky