Absence of Peroxiredoxin 6 Amplifies the Effect of Oxidant Stress on Mobility and SCSA/CMA3 Defined Chromatin Quality and Impairs Fertilizing Ability of Mouse Spermatozoa

Biology of Reproduction
Burak OzkosemCristian M O'Flaherty

Abstract

Oxidative stress, the imbalance between reactive oxygen species production and antioxidant defenses, is associated with male infertility. Peroxiredoxins (PRDXs) are antioxidant enzymes with a wide distribution in spermatozoa. PRDX6 is highly abundant and located in all subcellular compartments of the spermatozoon. Infertile men have lower levels of sperm PRDX6 associated with low sperm motility and high DNA damage. In order to better understand the role of PRDX6 in male reproduction, the aim of this study was to elucidate the impact of the lack of PRDX6 on male mouse fertility. Spermatozoa lacking PRDX6 showed significantly increased levels of cellular oxidative damage evidenced by high levels of lipid peroxidation, 8-hydroxy-deoxyguanosine (DNA oxidation), and protein oxidation (S-glutathionylation and carbonylation), lower sperm chromatin quality (high DNA fragmentation and low DNA compaction, due to low levels of protamination and a high percentage of free thiols), along with decreased sperm motility and impairment of capacitation as compared with wild-type (WT) spermatozoa. These manifestations of damage are exacerbated by tert-butyl hydroperoxide treatment in vivo. While WT males partially recovered the quality of their sp...Continue Reading

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Citations

Dec 28, 2016·Molecular Human Reproduction·Donghyun LeeCristian O'Flaherty
Sep 8, 2017·International Journal of Molecular Sciences·Do-Yeal RyuMyung-Geol Pang
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Jan 1, 2020·Antioxidants & Redox Signaling·Eleonora Scarlata, Cristian O'Flaherty
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Feb 26, 2020·Antioxidants·Pei You WuCristian O'Flaherty
Apr 3, 2020·Antioxidants·Cristian O'Flaherty
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Oct 30, 2021·Antioxidants & Redox Signaling·Steven Serafini, Cristian O'Flaherty

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

Sperm Vision HR
UnScanIt
WinList
Statistix
Northern Eclipse

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