Abstract
In the plant-pathogenic mollicute Spiroplasma citri, spiralin is the major lipoprotein at the cell surface and is thought to be one of the components involved in the interactions of the spiroplasma with its insect vector. With the aim of identifying surface proteins other than spiralin, monoclonal antibodies (mAbs) were produced by immunization of mice with the spiralin-defective S. citri mutant GII3-9a2. mAb 10G3 was found to react with several polypeptides of 43-47 and 80-95 kDa, all of which were detected in the detergent phase after Triton X-114 partitioning of proteins. Mass spectrometry (MALDI-TOF) analyses of the two major polypeptides P47 and P80 of GII3-9a2, reacting with mAb 10G3, revealed that P47 was a processed product and represented the C-terminal moiety of P80. Search for sequence homologies revealed that P80 shared strong similarities with the S. citri adhesion-related protein P89 (Sarp1) of S. citri BR3, and is one (named Scarp4a) of the eight Scarps encoded by the S. citri GII-3 genome. The eight scarp genes are carried by plasmids pSci1-5. Western immunoblotting of proteins with mAb 10G3 revealed that, in contrast to the insect-transmissible S. citri strain GII-3, the non-insect-transmissible strains ASP-1, ...Continue Reading
References
Apr 1, 1991·Journal of Bacteriology·C StamburskiJ M Bove
Nov 25, 1988·Nucleic Acids Research·F Corpet
Jan 1, 1986·Annales De L'Institut Pasteur. Microbiology·A FosR Vogel
Apr 1, 1993·In Vitro Cellular & Developmental Biology. Animal·G E Moore, D B Hood
Dec 1, 1996·Microbiology·X ChengJ Frey
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 6, 2000·Annual Review of Microbiology·I M LeeD E Gundersen-Rindal
Oct 24, 2000·Molecular Plant-microbe Interactions : MPMI·P GaurivaudJ M Bové
Sep 28, 2001·Gene·M BergJ Fletcher
May 4, 2002·Biochimica Et Biophysica Acta·Sabine CastanoHenri Wróblewski
Oct 18, 2002·Plasmid·Carole LartigueJoël Renaudin
Nov 12, 2002·Gene·Hisashi NishigawaShigetou Namba
Apr 5, 2003·Journal of Invertebrate Pathology·Elvan OzbekSaskia A Hogenhout
May 6, 2003·Annual Review of Phytopathology·Joseph M BovéMonique Garnier
Oct 9, 2003·Applied and Environmental Microbiology·Sybille DuretJoël Renaudin
Jul 9, 2004·Applied and Environmental Microbiology·A BoutareaudC Saillard
Jan 28, 2005·Molecular Plant-microbe Interactions : MPMI·Aurélie AndréJoël Renaudin
Mar 2, 2005·Plasmid·Robert E DavisJonathan Shao
Mar 11, 2005·Microbiology·Mark A PickettIan N Clarke
May 11, 2005·Chembiochem : a European Journal of Chemical Biology·Matthieu SainlosPierre Lehn
Oct 24, 2008·Phytopathology·Nabil KillinyColette Saillard
Oct 24, 2008·Phytopathology·J YuJ Fletcher
Oct 24, 2008·Phytopathology·M O KwonJ Fletcher
Oct 24, 2008·Phytopathology·J FletcherF Ye
Citations
Dec 2, 2011·Journal of Proteome Research·Dmitry AlexeevVadim Govorun
Dec 9, 2008·Molecular Plant-microbe Interactions : MPMI·Xiaodong BaiSaskia A Hogenhout
Mar 23, 2010·Applied and Environmental Microbiology·Marc BretonJoël Renaudin
Feb 2, 2010·Applied and Environmental Microbiology·Fabien LabroussaaColette Saillard
Apr 30, 2008·BMC Genomics·Colette SaillardXavier Foissac
Mar 3, 2011·PloS One·Fabien LabroussaaColette Saillard
Nov 3, 2012·PloS One·Laure BévenNathalie Arricau-Bouvery
Dec 7, 2010·Journal of Microbiological Methods·Marc BretonJoël Renaudin
Feb 16, 2010·Biologicals : Journal of the International Association of Biological Standardization·Gail E Gasparich
Jul 28, 2009·Gene·Yoshiko IshiiShigetou Namba
Aug 19, 2008·Molecular Plant Pathology·Saskia A HogenhoutShigetou Namba
Mar 24, 2016·BMC Microbiology·Marie-Pierre DubranaColette Saillard
Oct 4, 2008·Microbiology·Marc BretonJoël Renaudin
Apr 18, 2009·Microbiology·Yoshiko IshiiShigetou Namba
Dec 19, 2009·Microbiology·Sybille DuretNathalie Arricau-Bouvery
Apr 17, 2015·BMC Microbiology·Joël RenaudinXavier Foissac
Jan 25, 2018·Current Microbiology·Guo-Dong ZhaHan-Shou Yu
Sep 2, 2006·Microbiology·Nathalie BerhoJoël Renaudin
Nov 6, 2007·International Journal of Systematic and Evolutionary Microbiology·Daniel R BrownJanet M Bradbury
Mar 22, 2018·MBio·Florent MassonBruno Lemaitre
May 24, 2021·BMC Genomics·Rachel RattnerRaymond Yokomi