Absence of plasmids encoding adhesion-related proteins in non-insect-transmissible strains of Spiroplasma citri

Microbiology
Nathalie BerhoJoël Renaudin

Abstract

In the plant-pathogenic mollicute Spiroplasma citri, spiralin is the major lipoprotein at the cell surface and is thought to be one of the components involved in the interactions of the spiroplasma with its insect vector. With the aim of identifying surface proteins other than spiralin, monoclonal antibodies (mAbs) were produced by immunization of mice with the spiralin-defective S. citri mutant GII3-9a2. mAb 10G3 was found to react with several polypeptides of 43-47 and 80-95 kDa, all of which were detected in the detergent phase after Triton X-114 partitioning of proteins. Mass spectrometry (MALDI-TOF) analyses of the two major polypeptides P47 and P80 of GII3-9a2, reacting with mAb 10G3, revealed that P47 was a processed product and represented the C-terminal moiety of P80. Search for sequence homologies revealed that P80 shared strong similarities with the S. citri adhesion-related protein P89 (Sarp1) of S. citri BR3, and is one (named Scarp4a) of the eight Scarps encoded by the S. citri GII-3 genome. The eight scarp genes are carried by plasmids pSci1-5. Western immunoblotting of proteins with mAb 10G3 revealed that, in contrast to the insect-transmissible S. citri strain GII-3, the non-insect-transmissible strains ASP-1, ...Continue Reading

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Citations

Dec 9, 2008·Molecular Plant-microbe Interactions : MPMI·Xiaodong BaiSaskia A Hogenhout
Apr 30, 2008·BMC Genomics·Colette SaillardXavier Foissac
Dec 7, 2010·Journal of Microbiological Methods·Marc BretonJoël Renaudin
Feb 16, 2010·Biologicals : Journal of the International Association of Biological Standardization·Gail E Gasparich
Aug 19, 2008·Molecular Plant Pathology·Saskia A HogenhoutShigetou Namba
Mar 24, 2016·BMC Microbiology·Marie-Pierre DubranaColette Saillard
Jan 25, 2018·Current Microbiology·Guo-Dong ZhaHan-Shou Yu
Nov 6, 2007·International Journal of Systematic and Evolutionary Microbiology·Daniel R BrownJanet M Bradbury

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