Abstract
RstB/RstA is an uncharacterized Escherichia coli two-component system, the regulatory effects of which on the E. coli cell cycle remain unclear. We found that the doubling time and average number of replication origins per cell in an ΔrstB mutant were the same as the wild-type, and the average number of replication origins in an ΔrstA mutant was 18.2% lower than in wild-type cells. The doubling times were 34 min, 35 min, and 40 min for the wild-type, ΔrstB, and ΔrstA strains, respectively. Ectopic expression of RstA from plasmid pACYC-rstA partly reversed the ΔrstA mutant phenotypes. The amount of initiator protein DnaA per cell was reduced by 40% in the ΔrstA mutant compared with the wild-type, but the concentration of DnaA did not change as the total amount of cellular protein was also reduced in these cells. Deletion or overproduction of RstA does not change the temperature sensitivity of dnaA46, dnaB252 and dnaC2. The expression of hupA was decreased by 0.53-fold in ΔrstA. RstA interacted with Topoisomerase I weakly in vivo and increased its activity of relaxing the negative supercoiled plasmid. Our data suggest that deletion of RstA leads to delayed initiation of DNA replication, and RstA may affect initiation of replicati...Continue Reading
References
Jun 1, 1978·Journal of Bacteriology·A C Chang, S N Cohen
Feb 1, 1991·Research in Microbiology·E Boye, A Løbner-Olesen
Jun 2, 1989·Cell·A Løbner-OlesenE Boye
Jan 1, 1971·Molecular & General Genetics : MGG·J A Wechsler, J D Gross
Aug 1, 1984·Cell·J M Kaguni, A Kornberg
Sep 1, 1994·Journal of Bacteriology·L Brøndsted, T Atlung
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·G KarimovaD Ladant
Nov 3, 2001·Biochimica Et Biophysica Acta· MorigenA Løbner-Olesen
Oct 9, 2002·Molecular Microbiology·Valorie T RyanAlan C Leonard
Oct 9, 2002·Molecular Microbiology·Taku OshimaTakeshi Mizuno
Nov 29, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Marc DroletImad Baaklini
Sep 26, 2003·Molecular Microbiology· MorigenKirsten Skarstad
Mar 19, 2005·Journal of Bacteriology·Gouzel KarimovaDaniel Ladant
Jun 9, 2005·Journal of Bacteriology· MorigenKirsten Skarstad
Feb 14, 2006·Molecular Aspects of Medicine·Simone Fleige, Michael W Pfaffl
Jun 14, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Masanari KitagawaHirotada Mori
Feb 21, 2008·Molecular Microbiology·Sundari ChodavarapuJon M Kaguni
Mar 11, 2008·Microbial Cell Factories·Mihaela SkuljViktor Menart
Mar 8, 2012·PLoS Genetics·Norbert S HillPetra Anne Levin
Jun 12, 2012·Current Protocols in Pharmacology·John L NitissMargarita Mishina
Mar 29, 2014·PloS One·Feng Liu Morigen
Dec 19, 2015·Genetics and Molecular Research : GMR·Y YaoM Morigen
Dec 28, 2016·Genes·Yuan Yao Morigen