Absence of sodA Increases the Levels of Oxidation of Key Metabolic Determinants of Borrelia burgdorferi

PloS One
Maria D Esteve-GassentJ Seshu

Abstract

Borrelia burgdorferi, the causative agent of Lyme disease, alters its gene expression in response to environmental signals unique to its tick vector or vertebrate hosts. B. burgdorferi carries one superoxide dismutase gene (sodA) capable of controlling intracellular superoxide levels. Previously, sodA was shown to be essential for infection of B. burgdorferi in the C3H/HeN model of Lyme disease. We employed two-dimensional electrophoresis (2-DE) and immunoblot analysis with antibodies specific to carbonylated proteins to identify targets that were differentially oxidized in the soluble fractions of the sodA mutant compared to its isogenic parental control strain following treatment with an endogenous superoxide generator, methyl viologen (MV, paraquat). HPLC-ESI-MS/MS analysis of oxidized proteins revealed that several proteins of the glycolytic pathway (BB0057, BB0020, BB0348) exhibited increased carbonylation in the sodA mutant treated with MV. Levels of ATP and NAD/NADH were reduced in the sodA mutant compared with the parental strain following treatment with MV and could be attributed to increased levels of oxidation of proteins of the glycolytic pathway. In addition, a chaperone, HtpG (BB0560), and outer surface protein A ...Continue Reading

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Citations

Nov 24, 2017·BMC Microbiology·Christina M BrockMaria Esteve-Gasent
Aug 24, 2016·Infection and Immunity·Adrienne C ShowmanMollie W Jewett
May 6, 2017·Frontiers in Cellular and Infection Microbiology·Xuwu XiangYongliang Lou

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Methods Mentioned

BETA
Assay
Electrophoresis
fluorescence assay
PCR
protein folding

Software Mentioned

Proteome
Mascot
Scaffold
GPMAW

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