Absence of soluble leptin receptor in plasma from dbPas/dbPas and other db/db mice.

The Journal of Biological Chemistry
C LiJ M Friedman

Abstract

The leptin receptor (Ob-R) is alternatively spliced into at least five different RNAs designated Ob-R(a-e). Ob-R(a-d) predict receptors with a single transmembrane domain, and Ob-Re predicts a secreted form of the receptor. The presence of an approximately 120-kDa soluble leptin receptor in mouse plasma was confirmed by precipitation with leptin-Sepharose beads followed by immunobloting with anti-leptin receptor antibodies. The soluble leptin receptor is larger than that predicted by the primary sequence. Deglycosylation of the receptor with peptide N:glycosidase F results in a decrease in molecular mass to a size consistent with that of the primary sequence. The secreted receptor was present in plasma from wild type mice but was truncated in plasma from db3J/db3J and absent in dbPas/dbPas plasma. Although db3J/db3J mice are known to have a frameshift mutation at amino acid 625, the basis for the mutation in dbPas/dbPas mice was not known. Further studies indicated that dbPas/dbPas mice carry a duplication of exons 4 and 5 of Ob-R. This mutation introduces a premature stop codon into the protein at amino acid 281. The absence of Ob-R in db3J/db3J and dbPas/dbPas mice confirm the identify of the 120-kDa plasma protein as Ob-Re.

References

Jul 28, 1995·Science·M A PelleymounterF Collins
Feb 15, 1996·Nature·G H LeeJ M Friedman
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·N LevinF de Sauvage
Jan 1, 1997·The Journal of Clinical Investigation·P L GoldenW M Pardridge
Jun 1, 1997·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·F CuminN Levens
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·J L HalaasJ M Friedman
Jun 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·G H LeeJ M Friedman

❮ Previous
Next ❯

Citations

Jul 8, 2009·Cellular and Molecular Neurobiology·Olivia OsbornTamas Bartfai
Sep 12, 2000·Current Gastroenterology Reports·A KumarC D Still
May 15, 2002·European Journal of Pharmacology·Daniel W LeePatricia Grasso
Jun 16, 2000·Molecular and Cellular Endocrinology·G HorevM Friedman-Einat
Jun 1, 2004·Nature Medicine·Jeffrey M Friedman
Sep 1, 1999·Journal of Pediatric Gastroenterology and Nutrition·A G Hoppin, L M Kaplan
Jun 9, 2000·Annual Review of Physiology·R S Ahima, J S Flier
Feb 6, 2008·Proceedings of the American Thoracic Society·Alan R SchwartzPhilip L Smith
Nov 24, 2007·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·A W ThorburnJ Proietto
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·C Li, J M Friedman
Sep 15, 2014·Journal of Chemical Neuroanatomy·Nobuhiro WadaSeiji Shioda
Jul 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Wenwen ZengJeffrey M Friedman
May 9, 2009·British Journal of Pharmacology·Jiejin Zhang, Philip J Scarpace
Dec 12, 2013·Molecular Metabolism·Zhiying LiJeffrey Michael Friedman
Nov 24, 1999·Frontiers in Neuroendocrinology·F F Casanueva, C Dieguez
Feb 24, 2018·International Journal of Molecular Sciences·Chi-Ming WongMabel Yin-Chun Yau
Oct 31, 1998·Nature·J M Friedman, J L Halaas
Dec 5, 2000·The Journal of Biological Chemistry·L HuangC Li
Dec 13, 2006·Molecular Reproduction and Development·L T NicklinM G Hunter
Jan 13, 2005·The Journal of Biological Chemistry·Paul CohenCai Li
May 6, 2003·The Journal of Nutrition·Jung Han KimJürgen K Naggert
Jun 19, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·R B HarrisS M Redmann

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.