Absence of the genetic marker IS6110 from a strain of Mycobacterium tuberculosis isolated in Ontario.

The Canadian Journal of Infectious Diseases = Journal Canadien Des Maladies Infectieuses
S T HowardW M Johnson

Abstract

A 35-year-old female patient from Waterloo, Ontario was diagnosed with pulmonary tuberculosis in June 1995. Records indicated that the patient had emigrated from Laos circa 1990. A culture grown from a bronchoalveolar lavage specimen was identified as Mycobacterium tuberculosis by standard biochemical methods. Drug-susceptibility testing indicated the strain was resistant to pyrazinamide (PZA), and a mutation was detected within pncA, a gene associated with PZA resistance. Sequence data from the 16S rRNA gene and the 16S/23S rRNA gene spacer confirmed that the strain was a member of the M tuberculosis complex, and analysis of the mpcA and pncA genes supported the identification of the strain as M tuberculosis rather than Mycobacterium bovis. However, the insertion element IS6110, which is used for epidemiological tracing of M tuberculosis, was not detected in this strain by either restriction fragment length polymorphism analysis or by polymerase chain reaction. Two other genetic markers associated with the M tuberculosis complex, IS1081 and the direct repeat element, were present. The arrival of immigrants with tuberculosis from southeast Asia, where most strains of M tuberculosis lacking IS6110 have been traced, has important...Continue Reading

Citations

Aug 1, 2000·Indian Journal of Clinical Biochemistry : IJCB·M Sritharan, V Sritharan
Nov 9, 2012·Journal of Clinical Microbiology·Deborah SteenselsHans De Beenhouwer
Aug 3, 2002·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Catherine HankinsUNKNOWN SurvUDI Working Group
Sep 1, 2016·BMC Infectious Diseases·Sophia B GeorghiouTimothy C Rodwell

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