Absence of XMRV in peripheral blood mononuclear cells of ARV-treatment naïve HIV-1 infected and HIV-1/HCV coinfected individuals and blood donors.

PloS One
Cosmina GingarasJason T Kimata

Abstract

Xenotropic murine leukemia virus-related virus (XMRV) has been found in the prostatic tissue of prostate cancer patients and in the blood of chronic fatigue syndrome patients. However, numerous studies have found little to no trace of XMRV in different human cohorts. Based on evidence suggesting common transmission routes between XMRV and HIV-1, HIV-1 infected individuals may represent a high-risk group for XMRV infection and spread. DNA was isolated from the peripheral blood mononuclear cells (PBMCs) of 179 HIV-1 infected treatment naïve patients, 86 of which were coinfected with HCV, and 54 healthy blood donors. DNA was screened for XMRV provirus with two sensitive, published PCR assays targeting XMRV gag and env and one sensitive, published nested PCR assay targeting env. Detection of XMRV was confirmed by DNA sequencing. One of the 179 HIV-1 infected patients tested positive for gag by non-nested PCR whereas the two other assays did not detect XMRV in any specimen. All healthy blood donors were negative for XMRV proviral sequences. Sera from 23 HIV-1 infected patients (15 HCV(+)) and 12 healthy donors were screened for the presence of XMRV-reactive antibodies by Western blot. Thirteen sera (57%) from HIV-1(+) patients and 6...Continue Reading

References

Feb 1, 2003·Molecular Pathology : MP·P N NelsonP G Murray
Aug 7, 2003·Virus Research·Jean-Pierre MolèsJean-Jacques Guilhou
Oct 7, 2003·Nature Medicine·Mariana MarinDavid Kabat
Jan 18, 2008·Nature·Stuart J D NeilPaul D Bieniasz
Oct 1, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Nicole FischerThorsten Schlomm
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Robert SchlabergIla R Singh
Dec 5, 2009·Virology·Ryuta SakumaYasuhiro Ikeda
Jan 13, 2010·PloS One·Otto ErlweinAnthony Cleare
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Harriet C T GroomKate N Bishop
May 29, 2010·Emerging Infectious Diseases·Nicole FischerMartin Aepfelbacher
Jun 26, 2010·BMC Cancer·Margarita L Martinez-FierroAugusto Rojas-Martinez
Jul 20, 2010·International Reviews of Immunology·Eva BaladaJosep Ordi-Ros
Aug 14, 2010·PloS One·Marion CornelissenAntoinette C van der Kuyl
Aug 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shyh-Ching LoHarvey J Alter
Oct 23, 2010·Cancer Research·Amanda L AloiaAlan Rein
Dec 22, 2010·Retrovirology·Mark J RobinsonMyra O McClure
Dec 22, 2010·Retrovirology·Stéphane HuéGreg J Towers

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Citations

Apr 1, 2014·Emerging Microbes & Infections·Maribel Arias, Hung Fan
Jul 24, 2012·Current Opinion in Virology·Krista Delviks-FrankenberryVinay K Pathak
Jun 14, 2012·Transfusion·Simone A GlynnUNKNOWN NHLBI Emerging Infectious Disease Task Force convened November 7, 2011

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Datasets Mentioned

BETA
JN235142

Methods Mentioned

BETA
PCR
Genotyping
blood collection
PCRs

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