Absolute quantification of human liver metabolite concentrations by localized in vivo 31P NMR spectroscopy in diffuse liver disease

European Radiology
Bengt NorénOrjan Smedby

Abstract

Phosphorus-31 NMR spectroscopy using slice selection (DRESS) was used to investigate the absolute concentrations of metabolites in the human liver. Absolute concentrations provide more specific biochemical information compared to spectrum integral ratios. Nine patients with histopathologically proven diffuse liver disease and 12 healthy individuals were examined in a 1.5-T MR scanner (GE Signa LX Echospeed plus). The metabolite concentration quantification procedures included: (1) determination of optimal depth for the in vivo measurements, (2) mapping the detection coil characteristics, (3) calculation of selected slice and liver volume ratios using simple segmentation procedures and (4) spectral analysis in the time domain. The patients had significantly lower concentrations of phosphodiesters (PDE), 6.3+/-3.9 mM, and ATP-beta, 3.6+/-1.1 mM, (P<0.05) compared with the control group (10.0+/-4.2 mM and 4.2+/-0.3 mM, respectively). The concentrations of phosphomonoesters (PME) were higher in the patient group, although this was not significant. Constructing an anabolic charge (AC) based on absolute concentrations, [PME]/([PME] + [PDE]), the patients had a significantly larger AC than the control subjects, 0.29 vs. 0.16 (P<0.005)...Continue Reading

References

Dec 4, 1978·Biochimica Et Biophysica Acta·B de KruijffL L van Deenen
Mar 1, 1992·Journal of Magnetic Resonance Imaging : JMRI·V RajanayagamB D Ross
Dec 1, 1990·Analytical Biochemistry·P LundbergH J Vogel
Oct 1, 1989·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·T E BatesD G Gadian
Nov 1, 1989·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·E J MurphyG K Radda
Jan 1, 1988·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J W van der VeenD van Ormondt
Oct 17, 1995·Biochimica Et Biophysica Acta·S D Taylor-RobinsonJ D Bell
Feb 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D K MenonM Y Morgan
Feb 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H N van Wassenaer-van HallW P Mali
Oct 1, 1994·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·R BuchliP Boesiger
Oct 1, 1994·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·R BuchliP Boesiger
Nov 25, 1993·Biochimica Et Biophysica Acta·J D BellJ Urenjak
May 1, 1993·NMR in Biomedicine·G B MatsonM W Weiner
Jan 1, 1996·Progress in Biophysics and Molecular Biology·I J Cox
Nov 30, 1999·JAMA : the Journal of the American Medical Association·H Cortez-PintoA M Diehl
Feb 11, 2000·NMR in Biomedicine·P C DagnelieP v van Dijk
Feb 14, 2002·Magma·Maria LjungbergEva Forssell-Aronsson
Apr 2, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Adrian K P LimSimon D Taylor-Robinson
Feb 26, 2004·Computer Methods and Programs in Biomedicine·R de BeerD van Ormondt

❮ Previous
Next ❯

Citations

Jun 9, 2009·European Radiology·Paul E Sijens
Feb 12, 2013·Seminars in Ultrasound, CT, and MR·Edmund M GodfreyDavid J Lomas
Dec 23, 2011·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Xi-an Mao
Jun 27, 2013·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Alessandra LaufsJong-Hee Hwang
Apr 3, 2016·NMR in Biomedicine·J LöringD W J Klomp
Nov 22, 2008·Journal of Hepatology·Susanne BonekampJeanne Clark
Apr 29, 2011·Diabetes·Douglas E Befroy, Gerald I Shulman
Jan 26, 2017·Analytical Biochemistry·Ladislav ValkovičMartin Krššák
Jun 24, 2017·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Andreas Korzowski, Peter Bachert
Dec 8, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ganeko Bernardo-SeisdedosOscar Millet

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.