Absolute quantification of metabolites in breast cancer cell extracts by quantitative 2D (1) H INADEQUATE NMR

NMR in Biomedicine
Estelle MartineauPatrick Giraudeau

Abstract

Metabolomic studies by NMR spectroscopy are increasingly employed for a variety of biomedical applications. A very standardized 1D proton NMR protocol is generally employed for data acquisition, associated with multivariate statistical tests. Even if targeted approaches have been proposed to quantify metabolites from such experiments, quantification is often made difficult by the high degree of overlap characterizing (1) H NMR spectra of biological samples. Two-dimensional spectroscopy presents a high potential for accurately measuring concentrations in complex samples, as it offers a much higher discrimination between metabolite resonances. We have recently proposed an original approach relying on the (1) H 2D INADEQUATE pulse sequence, optimized for fast quantitative analysis of complex metabolic mixtures. Here, the first application of the quantitative (1) H 2D INADEQUATE experiment to a real metabonomic study is presented. Absolute metabolite concentrations are determined for different breast cancer cell line extracts, by a standard addition procedure. The protocol is characterized by high analytical performances (accuracy better than 1%, excellent linearity), even if it is affected by relatively long acquisition durations ...Continue Reading

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Citations

Jul 12, 2014·Annual Review of Analytical Chemistry·Patrick Giraudeau, Lucio Frydman
Feb 24, 2016·Analytical Chemistry·Niels K J HermkensMarco Tessari
Aug 15, 2014·The Journal of Organic Chemistry·Israel FernándezAntonio Herrera
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Aug 21, 2013·Journal of Pharmaceutical and Biomedical Analysis·Patrick GiraudeauSerge Akoka
Feb 17, 2015·Journal of Advanced Research·Engy A Mahrous, Mohamed A Farag
Feb 28, 2015·Journal of Pharmaceutical and Biomedical Analysis·Estelle MartineauPatrick Giraudeau
Dec 6, 2014·The Journal of Organic Chemistry·María Encarnación Fernández-ValleAntonio Herrera
May 18, 2016·Expert Opinion on Drug Discovery·Suruchi Singh, Raja Roy
Apr 16, 2013·Analytical Chemistry·Estelle MartineauPatrick Giraudeau
May 27, 2014·Analytical Chemistry·Adrien Le GuennecStefano Caldarelli
Nov 8, 2013·Analytical Chemistry·Kerem Bingol, Rafael Brüschweiler
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Apr 3, 2020·Magnetic Resonance in Chemistry : MRC·Estelle MartineauPatrick Giraudeau
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Jul 3, 2016·Magnetic Resonance in Chemistry : MRC·Patrick Giraudeau
Jul 18, 2017·Analytical Chemistry·Martina Palomino-SchätzleinMíriam Pérez-Trujillo
Jan 6, 2018·Analytical Chemistry·Jonathan FarjonPatrick Giraudeau
Dec 11, 2020·Analytical Chemistry·Adilah BahadoorJeremy E Melanson

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