Absolute transcript levels of thioredoxin- and glutathione-dependent redox systems in Saccharomyces cerevisiae: response to stress and modulation with growth

The Biochemical Journal
Fernando Monje-CasasCarmen Pueyo

Abstract

We report the co-ordinated fine-tune of mRNA molecules that takes place in yeast (Saccharomyces cerevisiae) in response to diverse environmental stimuli. We performed a systematic and refined quantification of the absolute expression patterns of 16 genes coding for thioredoxin- and glutathione-dependent redox system components. Quantifications were performed to examine the response to oxidants, to sudden temperature upshifts and in association with metabolic changes accompanying culture growth and to explore the contribution of mRNA decay rates to the differences observed in basal expression levels. Collectively, these quantifications show (i) vast differences in the steady-state amounts of the investigated transcripts, cTPxI being largely overexpressed compared with GPX1 during the exponential phase and GPX2 beyond this growth stage; (ii) drastic changes in the relative abundance of the transcripts in response to oxidants and heat shock; and (iii) a unique temporal expression profile for each transcript as cells proceed from exponential to stationary growth phase, yet with some general trends such as maximal or near-maximal basal amounts of most mRNA species at early growth stages when glucose concentration is high and cells a...Continue Reading

References

Jul 1, 1977·Journal of Molecular Biology·D Kennell, H Riezman
Jan 1, 1991·Methods in Enzymology·R ParkerA Jacobson
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·I H KimS G Rhee
May 1, 1987·Molecular and Cellular Biology·M NonetR Young
Oct 1, 1973·Journal of Bacteriology·D J Tipper
Jun 1, 1973·Antimicrobial Agents and Chemotherapy·A JimenezJ Davies
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·V Iyer, K Struhl
Feb 22, 2000·The Journal of Biological Chemistry·S G ParkI H Kim
Dec 5, 2000·Molecular Biology of the Cell·A P GaschP O Brown
Feb 24, 2001·European Journal of Pediatrics·R G Cotton
Jul 11, 2001·The Journal of Biological Chemistry·A M Avery, S V Avery
Feb 1, 2002·The Journal of Biological Chemistry·Seung-Keun HongIl-Han Kim
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yulei WangPatrick O Brown
Nov 20, 2002·Cell·Agnès DelaunayMichel B Toledano
Mar 5, 2003·Free Radical Biology & Medicine·Chi-Ming WongDong-Yan Jin
Mar 13, 2003·Archives of Microbiology·Jeanette BrejningNils Arneborg
Sep 5, 2003·The Journal of Biological Chemistry·Juan JuradoCarmen Pueyo
Feb 12, 2008·Current Protocols in Molecular Biology·M A Collart, S Oliviero

❮ Previous
Next ❯

Citations

Oct 7, 2008·Applied Microbiology and Biotechnology·Roberto Pérez-TorradoEmilia Matallana
Mar 7, 2007·Journal of Biomolecular NMR·Gisele Cardoso AmorimFabio C L Almeida
Sep 4, 2012·Nucleic Acids Research·Elizabeth WiltroutTao Pan
Oct 13, 2007·Proteins·Anderson Sá PinheiroAna Paula Valente
May 4, 2005·Yeast
Jul 23, 2020·G3 : Genes - Genomes - Genetics·Eyal Metzl-RazNaama Barkai
Nov 18, 2020·FEMS Yeast Research·Monika BorkowskaEwelina Celińska
Feb 6, 2020·Biochemistry and Biophysics Reports·Claudia Patricia Bravo-ChaucanésJoão Alexandre Ribeiro Gonçalves Barbosa
Jun 22, 2006·Environmental Science & Technology·Julia Ruiz-LagunaCarmen Pueyo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.