PMID: 11901089Mar 20, 2002Paper

Absorption and metabolism of flavonoids in the caco-2 cell culture model and a perused rat intestinal model

Drug Metabolism and Disposition : the Biological Fate of Chemicals
Yan Liu, Ming Hu

Abstract

The purpose of present study was to determine the intestinal absorption and metabolism of genistein and its analogs to better understand the mechanisms responsible for their low oral bioavailability. The Caco-2 cell culture model and a perfused rat intestinal model were used for the study. In both models, permeabilities of aglycones (e.g., genistein) were comparable to well absorbed compounds, such as testosterone and propranolol. In the Caco-2 model, permeabilities of aglycones were at least 5 times higher (p < 0.05) than their corresponding glycosides (e.g., genistin), and the vectorial transport of aglycones was similar (p > 0.05). In contrast, vectorial transport of glucosides favored excretion (p < 0.05). Limited hydrolysis of glycosides was observed in the Caco-2 model, which was completely inhibited (p < 0.05) by 20 mM gluconolactone, a broad specificity glycosidase inhibitor. In the perfused rat intestinal model, genistin was rapidly hydrolyzed (about 40% in 15 min) in the upper intestine but was not hydrolyzed at all in the colon. Aglycones were rapidly absorbed (P*(eff) > 1.5), and absorbed aglycones underwent extensive (40% maximum) phase II metabolism via glucuronidation and sulfation in the upper small intestine. S...Continue Reading

References

Dec 1, 1991·Pharmaceutical Research·R A ConradiP S Burton
Sep 1, 1995·The Journal of Nutrition·X XuS Hendrich
Jan 1, 1996·The Journal of Nutrition·R A KingR J Head
Jan 1, 1997·Annual Review of Nutrition·M S Kurzer, X Xu
May 16, 1998·The American Journal of Clinical Nutrition·R A King, D B Bursill
Aug 28, 1998·Bioscience, Biotechnology, and Biochemistry·K IokuJ Terao
May 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·A D MottinoM Vore
Sep 25, 2001·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A Ayrton, P Morgan

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Citations

Jan 16, 2013·Applied Microbiology and Biotechnology·Shen-Shih Chiang, Tzu-Ming Pan
Jul 20, 2005·Pharmaceutical Research·Li ZhangZhong Zuo
Oct 29, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dafang ZhongJinghua Xu
Feb 25, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Claudio GardanaPaolo Simonetti
Jun 24, 2008·Critical Reviews in Food Science and Nutrition·Theresa LarkinLee Astheimer
Mar 29, 2008·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Stephen W J WangMing Hu
Jun 10, 2011·Annals of Nutrition & Metabolism·G Sarwar GilaniGerard M Cooke
Apr 3, 2012·Chemical & Pharmaceutical Bulletin·Dun WangXin Yu
Jan 15, 2014·Journal of Natural Products·Jatinder Kaur MukkerJane Alcorn
Jul 9, 2013·Journal of Pharmacological and Toxicological Methods·Zhiqiang LuoLi Wei
Jan 31, 2014·Journal of Medicinal Food·Chukwuemezie ChimezieRobert E Stratford
Oct 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nora S GreenJeffery W Kelly
Dec 26, 2015·International Journal of Environmental Research and Public Health·Chandler Schexnayder, Robert E Stratford
Jun 2, 2007·Expert Opinion on Drug Metabolism & Toxicology·Zhongqiu Liu, Ming Hu

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