Absorption and metabolism of orally administered collagen hydrolysates evaluated by the vascularly perfused rat intestine and liver in situ

Biomedical Research
Yoshihiro OsawaYukihito Kabuyama

Abstract

A number of studies have shown that oral administration of collagen hydrolysate (CH) results in the absorption of di- and tri-peptides. In order to understand the dynamics of CH absorption and metabolism, molecular profiles of hydroxyproline (Hyp) and Hyp-containing peptides (HCPs) were analyzed by in situ perfusion of rat intestine and liver. The total amount of absorbed HCPs during 1 h of perfusion was 16.6 μmol, which was significantly higher than that of free Hyp (6.6 μmol). In addition, HCPs were also reliably detected in hepatic perfusate at the level higher than free Hyp. Thus, the results demonstrated that CH is absorbed predominantly as peptides, which subsequently enter systemic circulation. Size exclusion chromatography showed that perfusates include significant amount of HCPs larger than tripeptides, leading us to analyze these peptides in detail. Mass spectrometric analysis of intestinal perfusate finally identified three CH-derived peptides, which are surprisingly large as food-derived circulating peptides. Peptide quantitation by liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that di- and tri-peptides, which are previously identified as major peptides in circulating blood, comprise only a part...Continue Reading

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Citations

Feb 16, 2019·Journal of Food Science·Carlos A López-MoralesEmilio Medina-Rivero
Jul 2, 2019·Journal of Nutritional Science and Vitaminology·Takafumi MizushigeYukihito Kabuyama
Mar 3, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dai NogimuraYukihito Kabuyama
Feb 27, 2020·Antioxidants·Gabriel Aguirre-CruzGabriel Aguirre-Álvarez
Jul 13, 2020·Biochemical Pharmacology·Gabor TajtiRaymond S Norton
Oct 31, 2019·Analytical Chemistry·Said Vázquez-LeyvaEmilio Medina-Rivero

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