Absorption by water increases fluorescence image contrast of biological tissue in the shortwave infrared

Proceedings of the National Academy of Sciences of the United States of America
Jessica A CarrMoungi G Bawendi

Abstract

Recent technology developments have expanded the wavelength window for biological fluorescence imaging into the shortwave infrared. We show here a mechanistic understanding of how drastic changes in fluorescence imaging contrast can arise from slight changes of imaging wavelength in the shortwave infrared. We demonstrate, in 3D tissue phantoms and in vivo in mice, that light absorption by water within biological tissue increases image contrast due to attenuation of background and highly scattered light. Wavelengths of strong tissue absorption have conventionally been avoided in fluorescence imaging to maximize photon penetration depth and photon collection, yet we demonstrate that imaging at the peak absorbance of water (near 1,450 nm) results in the highest image contrast in the shortwave infrared. Furthermore, we show, through microscopy of highly labeled ex vivo biological tissue, that the contrast improvement from water absorption enables resolution of deeper structures, resulting in a higher imaging penetration depth. We then illustrate these findings in a theoretical model. Our results suggest that the wavelength-dependent absorptivity of water is the dominant optical property contributing to image contrast, and is theref...Continue Reading

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Citations

Mar 10, 2019·Journal of Biomedical Optics·Brook K ByrdScott C Davis
Dec 12, 2018·Photochemistry and Photobiology·Alexander Alabugin
May 13, 2020·Expert Opinion on Drug Discovery·Maria J MorenoDanica B Stanimirovic
Sep 29, 2019·Journal of Cellular and Molecular Medicine·Leyla Fath-BayatiEhsan Sharif-Paghaleh
Jul 1, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Miao FengRuichan Lv
Oct 10, 2020·The Analyst·Ruochong ZhangYuanjin Zheng
Feb 16, 2020·Cell·Shan ZhaoAli Ertürk
Apr 23, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Pascal M GschwendSotiris E Pratsinis
May 4, 2021·Journal of the American Chemical Society·Emily D CoscoEllen M Sletten
Jun 12, 2021·ACS Biomaterials Science & Engineering·Suman BishnoiSharad Gupta
Jul 30, 2019·Journal of the American Chemical Society·Wei ChenEllen M Sletten
Jan 24, 2020·ACS Applied Materials & Interfaces·Jian DuBen Zhong Tang
Sep 26, 2021·Light, Science & Applications·Zhe FengJun Qian

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