Absorption of 2-hydroxy-4-methylthiobutyrate and conversion to methionine in lambs

Journal of Dairy Science
G E LobleyM Vázquez-Añón

Abstract

Absorption and metabolism of the Met hydroxy analog 2-hydroxy-4-methylthiobutyrate (HMTBA) was examined using stable isotopes. In the first trial, Dl[1-13C]HMTBA was infused for 6 h (7.4 micromol/min) into the abomasum, and [2H3]Met was infused into the mesenteric vein, of 4 lambs prepared with vascular catheters across the splanchnic bed. Daily, lambs were offered 35 g of a mixed forage-concentrate feed/kg. Recovery of HMTBA at the portal vein was 87%, and of this, 63% bypassed the liver. In contrast, hepatic extraction of Met equaled or exceeded net absorption. Only small quantities of Met synthesized from HMTBA were exported from either the digestive tract or liver, but there was substantial and significant input from posthepatic tissues. In a second experiment, 3 of the lambs were killed following 4-h infusions of DL[1-13C]HMTBA and [2H3]Met with enrichments monitored in 15 tissues. Only kidney showed [1-13C]Met enrichment higher than plasma, which suggests that it must be a primary source of plasma Met derived from HMTBA. Based on comparison of plasma and intracellular [1-13C]:[2H3]Met enrichments, all tissues synthesized Met from HMTBA but to significantly different extents. The lowest values were for muscle, skin, brain,...Continue Reading

References

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Jan 1, 1994·The British Journal of Nutrition·G E LobleyT A Ewing
May 1, 1995·The British Journal of Nutrition·G E LobleyD A Farningham
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Mar 2, 2006·Journal of Dairy Science·T J WesterG E Lobley

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Citations

May 23, 2007·Journal of Dairy Science·H LapierreG E Lobley
Feb 23, 2011·Journal of Dairy Science·H LapierreG E Lobley
Dec 5, 2006·Current Opinion in Clinical Nutrition and Metabolic Care·Douglas G Burrin, Barbara Stoll
Aug 11, 2006·Journal of Dairy Science·G E LobleyM Vázquez-Añón
Dec 24, 2011·Journal of Dairy Science·H LapierreG E Lobley
Oct 20, 2009·Nutrition Research Reviews·Caroline Bauchart-ThevretDouglas G Burrin
Mar 2, 2006·Journal of Dairy Science·T J WesterG E Lobley
Aug 6, 2019·BioMed Research International·Fangrui ZuoJian Peng

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