Absorption of Rickettsia rickettsii antibodies by Rickettsia rickettsii antigens in four diagnostic tests.

Journal of Clinical Microbiology
K E HechemyH A Gaafar

Abstract

Immunoabsorption profiles were determined for Rickettsia rickettsii antigens used in four confirmatory tests for detection of antibodies to Rocky Mountain spotted fever. A human serum reactive in the four tests was absorbed with each test antigen and then reexamined by all four tests. The results indicated that the whole organism and complement-fixation antigen absorbed the whole array of antibodies to R. rickettsii, whereas erythrocyte-sensitizing substance coated on latex or erythrocytes did not. With erythrocyte-sensitizing substance coated on latex there was a decrease in titer when tested by the latex, indirect hemagglutination, and complement fixation tests but none when tested by the microimmunofluorescence test. With erythrocyte-sensitizing substance coated on sheep erythrocytes there was a decrease in titer when tested by indirect hemagglutination or complement fixation but none when tested by latex or microimmunofluorescence. These findings confirm that erythrocyte-sensitizing substance contains more than a single antigenic substance and plays a major role in the complement fixation test and a minor role in the microimmunofluorescence test.

References

Jan 1, 1977·American Journal of Epidemiology·R N PhilipS Vick
Jun 1, 1979·Infection and Immunity·J R MurphyC L Wisseman
Jun 1, 1975·Infection and Immunity·R L AnackerW D Bickel
Aug 1, 1974·Applied Microbiology·K HechemyH A Gaafar
Aug 1, 1980·Journal of Clinical Microbiology·K E HechemyE E Michaelson

❮ Previous
Next ❯

Citations

May 18, 2001·The New England Journal of Medicine·D RaoultT J Marrie
Feb 2, 2006·Scandinavian Journal of Infectious Diseases·Mogens JenseniusBjørn Myrvang
May 27, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mogens JenseniusUNKNOWN Norwegian African Tick Bite Fever Study Group
Jun 25, 2013·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·K NilssonC Påhlson
Jun 8, 2001·Journal of the American Veterinary Medical Association·J A ComerJ E Childs
Jul 10, 2004·Clinical and Diagnostic Laboratory Immunology·Mogens JenseniusDidier Raoult
Oct 1, 1983·Journal of Clinical Microbiology·K E HechemyL B Elliott
Aug 20, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M JenseniusB Myrvang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Complement Activation

The complement system can be activated by antigen-associated antibody. In the classical pathway of complement activation, C1q, C4b, and C3b are all able to bind to the Fc portion of IgG or IgM. Find the latest research on antibodies and complement activation here.