Abundance and distribution of RNA polymerase II in Arabidopsis interphase nuclei

Journal of Experimental Botany
Veit Schubert, Klaus Weisshart

Abstract

RNA polymerase II (RNAPII) is responsible for the transcription of most eukaryotic protein-coding genes. Analysing the topological distribution and quantification of RNAPII can contribute to understanding its function in interphase nuclei. Previously it was shown that RNAPII molecules in plant nuclei form reticulate structures within euchromatin of differentiated Arabidopsis thaliana nuclei rather than being organized in distinct 'transcription factories' as observed in mammalian nuclei. Immunosignal intensity measurements based on specific antibody labelling in maximum intensity projections of image stacks acquired by structured illumination microscopy (SIM) suggested a relative proportional increase of RNAPII in endopolyploid plant nuclei. Here, photoactivated localization microscopy (PALM) was applied to determine the absolute number and distribution of active and inactive RNAPII molecules in differentiated A. thaliana nuclei. The proportional increase of RNAPII during endopolyploidization is confirmed, but it is also shown that PALM measurements are more reliable than those based on SIM in terms of quantification. The single molecule localization results show that, although RNAPII molecules are globally dispersed within pla...Continue Reading

References

Oct 28, 1976·Chromosoma·C D Laird, W Y Chooi
Oct 8, 1998·The Journal of Cell Biology·R AbranchesP J Shaw
Dec 28, 1999·Trends in Cell Biology·R D Kornberg
Dec 29, 2000·Nature·UNKNOWN Arabidopsis Genome Initiative
Apr 20, 2002·Biophysical Journal·Russell E ThompsonWatt W Webb
Sep 16, 2003·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Sonya Martin, Ana Pombo
May 18, 2004·Current Opinion in Cell Biology·Robert J SimsDanny Reinberg
Sep 14, 2004·Nature Genetics·Cameron S OsbornePeter Fraser
Apr 5, 2005·Nature Genetics·Markus SchmidJan U Lohmann
May 11, 2005·Nature Reviews. Genetics·Mads KaernJames J Collins
Oct 6, 2005·Genetics·Daniel J KliebensteinDina A St Clair
Dec 20, 2005·Cell·Ido GoldingEdward C Cox
Jan 19, 2006·The Journal of Cell Biology·Jill M BrownVeronica J Buckle
Oct 20, 2006·PLoS Biology·Arjun RajSanjay Tyagi
Nov 24, 2006·Nature·Alex SigalUri Alon
May 7, 2008·Trends in Genetics : TIG·Sylvain Egloff, Shona Murphy
May 23, 2008·Journal of Cell Science·Christopher H EskiwPeter R Cook
Sep 24, 2008·The Journal of Cell Biology·Jill M BrownVeronica J Buckle
Nov 18, 2008·Nature Structural & Molecular Biology·Daniel ZenklusenRobert H Singer
Apr 22, 2009·PloS One·Ariel Aharon CohenUri Alon
Jan 7, 2010·PLoS Biology·Carmelo FerraiMassimo P Crippa
Feb 19, 2010·Nature·Arjun RajAlexander van Oudenaarden
Jul 30, 2010·Cold Spring Harbor Perspectives in Biology·Lyubomira Chakalova, Peter Fraser
Dec 7, 2010·Nature Structural & Molecular Biology·Saumil J GandhiRobert H Singer
Jul 13, 2011·Nucleic Acids Research·M ReiterM W Pfaffl
Jul 22, 2011·Nature·Gene-Wei Li, X Sunney Xie
Nov 3, 2011·Journal of Cell Science·Christopher H Eskiw, Peter Fraser
Nov 19, 2011·Chromosoma·Chunhui Hou, Victor G Corces
Mar 1, 2012·Current Opinion in Genetics & Development·Lucas Brandon Edelman, Peter Fraser
Apr 14, 2012·F1000 Biology Reports·Johan H Gibcus, Job Dekker
Apr 26, 2012·PLoS Genetics·Christina L RichardsMichael D Purugganan

❮ Previous
Next ❯

Citations

Dec 10, 2015·Epigenetics : Official Journal of the DNA Methylation Society·Iva MozgováLars Hennig
Oct 21, 2015·Trends in Plant Science·George KomisJozef Šamaj
Aug 22, 2015·Genome Biology·Chang Liu, Detlef Weigel
Feb 10, 2018·Methods and Applications in Fluorescence·J VangindertaelK P F Janssen
Mar 15, 2019·Nucleus·Ariadna Picart-PicoloFrédéric Pontvianne
Jun 20, 2020·Journal of Experimental Botany·Ana Paula SantosSara Farrona
Jul 9, 2020·Journal of Experimental Botany·Ying HuangJuan Sebastian Ramirez-Prado
Jul 10, 2019·Frontiers in Plant Science·Mateusz ZelkowskiVeit Schubert
Aug 9, 2019·Plant Biotechnology Journal·Mahdi Moradpour, Siti Nor Akmar Abdulah
Jul 5, 2018·Frontiers in Plant Science·Christophe Lambing, Stefan Heckmann
Jun 26, 2020·Frontiers in Plant Science·Tereza VavrdováGeorge Komis
Apr 30, 2017·Frontiers in Plant Science·Veit Schubert
Sep 18, 2020·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Ivona KubalováVeit Schubert
Mar 7, 2021·International Journal of Molecular Sciences·Ivona KubalováVeit Schubert
Jul 25, 2021·International Journal of Molecular Sciences·Adriana VolnáJiří Červeň
Nov 11, 2017·Chemical Reviews·Rainer Heintzmann, Thomas Huser

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
imaging technique
fluorescence microscopy
confocal microscopy
electron microscopy
super-resolution microscopy
FACS
Hi-C
Genome Sequencing

Software Mentioned

ZEN Channel
Excel
ZEN
SigmaPlot
RNAPIISer2ph
PRILM
SIM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Cell Science. Supplement
T A HughesP R Cook
Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character
J CHAYENU J MILES
Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Michael GuéThomas Boudier
© 2022 Meta ULC. All rights reserved