Abundance and diversity of prokaryotes in ephemeral hypersaline lake Chott El Jerid using Illumina Miseq sequencing, DGGE and qPCR assays

Extremophiles : Life Under Extreme Conditions
Manel Ben AbdallahSami Sayadi

Abstract

Chott El Jerid is the largest hypersaline ephemeral lake in southern Tunisian Sahara desert and is one of the biggest depressions at the North of Africa. This study aimed to investigate the diversity and abundance of microbial communities inhabiting Chott El Jerid during wet season (when it was flooded), using molecular methods [Illumina Miseq sequencing, DGGE and qPCR (qPCR)]. 16S rRNA gene analyses revealed that bacterial community was dominated by Proteobacteria (especially Ralstonia species), followed by Firmicutes, Bacteroidetes, Cyanobacteria, Actinobacteria and Verrucomicrobia. The results obtained using prokaryotic universal primers showed low relative abundance of Archaea dominated by few OTUs related to Methanosarcinaceae and Methanomassiliicoccaceae families and the presence of sulfate-reducing Archaea affiliated with Archaeoglobus. However, the results obtained using Archaea-specific primers showed that archaeal community was mainly composed of aerobic Halobacteria (especially Halorubrum species) and anaerobic members of Methanomicrobia. These results also provided evidence for the presence of members of the genus Halohasta in this environment. qPCR results revealed that Archaea were more abundant in studied samples...Continue Reading

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Citations

Feb 13, 2021·Archaea : an International Microbiological Journal·Fatma KarraySami Sayadi
Sep 1, 2020·The Science of the Total Environment·Oumayma KahlaAsma Sakka Hlaili

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Datasets Mentioned

BETA
KX009933
KX009953
MG880721
MG880736
KX009954
KX010007
MG250581
MG250596
MG856364
MG856382

Methods Mentioned

BETA
PCR
Assay
electrophoresis
Illumina sequencing

Software Mentioned

blast
Illumina
Illumina Miseq
EMBOSS
QIIME
MEGA
MUSCLE
Miseq
Qpcr

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