Abundance and dynamics of antibiotic resistance genes and integrons in lake sediment microcosms

PloS One
Björn BerglundPer-Eric Lindgren

Abstract

Antibiotic resistance in bacteria causing disease is an ever growing threat to the world. Recently, environmental bacteria have become established as important both as sources of antibiotic resistance genes and in disseminating resistance genes. Low levels of antibiotics and other pharmaceuticals are regularly released into water environments via wastewater, and the concern is that such environmental contamination may serve to create hotspots for antibiotic resistance gene selection and dissemination. In this study, microcosms were created from water and sediments gathered from a lake in Sweden only lightly affected by human activities. The microcosms were exposed to a mixture of antibiotics of varying environmentally relevant concentrations (i.e., concentrations commonly encountered in wastewaters) in order to investigate the effect of low levels of antibiotics on antibiotic resistance gene abundances and dynamics in a previously uncontaminated environment. Antibiotic concentrations were measured using liquid chromatography-tandem mass spectrometry. Abundances of seven antibiotic resistance genes and the class 1 integron integrase gene, intI1, were quantified using real-time PCR. Resistance genes sulI and ermB were quantified ...Continue Reading

References

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Jan 23, 2014·The Science of the Total Environment·Björn BerglundPer-Eric Lindgren

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Citations

Nov 11, 2016·Open Biology·Carolyn A MichaelMaurizio Labbate
Mar 23, 2018·International Journal of Environmental Research and Public Health·Wenxia WangZhigang Mao
Oct 2, 2019·Water Research·Richard L CarneyJustin R Seymour
Aug 31, 2017·Environmental Science & Technology·Ying HanGuangfei Liu

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Methods Mentioned

BETA
PCR
PCRs

Software Mentioned

Prism

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