Abundance of Antibiotic Resistance Genes in Bacteriophage following Soil Fertilization with Dairy Manure or Municipal Biosolids, and Evidence for Potential Transduction

Applied and Environmental Microbiology
Joseph Ross, Edward Topp

Abstract

Animal manures and municipal biosolids recycled onto crop production land carry antibiotic-resistant bacteria that can influence the antibiotic resistome of agricultural soils, but little is known about the contribution of bacteriophage to the dissemination of antibiotic resistance genes (ARGs) in this context. In this work, we quantified a set of ARGs in the bacterial and bacteriophage fractions of agricultural soil by quantitative PCR. All tested ARGs were present in both the bacterial and phage fractions. We demonstrate that fertilization of soil with dairy manure or human biosolids increases ARG abundance in the bacterial fraction but not the bacteriophage fraction and further show that pretreatment of dairy manure can impact ARG abundance in the bacterial fraction. Finally, we show that purified bacteriophage can confer increased antibiotic resistance to soil bacteria when combined with selective pressure. The results indicate that soilborne bacteriophage represents a substantial reservoir of antibiotic resistance and that bacteriophage could play a significant role in the horizontal transfer of resistance genes in the context of an agricultural soil microbiome. Overall, our work reinforces the advisability of composting o...Continue Reading

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Citations

Jul 15, 2016·Environmental Science & Technology·Hsiao-Ying ChengJonathan J Silberg
Oct 22, 2016·Annals of the New York Academy of Sciences·Michael R GillingsSasha G Tetu
Oct 22, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Matthias Willmann, Silke Peter
Apr 20, 2017·Frontiers in Microbiology·Ferran Navarro, Maite Muniesa
Feb 7, 2018·Frontiers in Microbiology·Weilong ZhouZhiyong Zong
Dec 6, 2017·Antibiotics·Lorena Rodríguez-RubioMaite Muniesa
Jan 15, 2019·Journal of Environmental Quality·Danika N HillMatthew J Morra
Oct 12, 2018·Emerging Microbes & Infections·Clara Torres-Barceló
Jan 24, 2018·FEMS Microbiology Ecology·Chad W McKinneyApril B Leytem
Feb 1, 2020·International Microbiology : the Official Journal of the Spanish Society for Microbiology·José Luis Balcázar
Nov 20, 2016·Annals of the New York Academy of Sciences·José L MartínezFernando Baquero
Jan 17, 2019·The Journal of Antimicrobial Chemotherapy·Dietmar Fernández-OrthFerran Navarro
Feb 6, 2021·The Science of the Total Environment·Xi-Ran WangJian Sun
Nov 28, 2020·The Science of the Total Environment·Min XuJames M Tiedje
Jan 5, 2021·Canadian Journal of Microbiology·Juan Martín Talavera-GonzálezCelene Salgado-Miranda
Mar 3, 2020·The Science of the Total Environment·Yashini NaidooDon A Cowan
May 11, 2021·Frontiers in Microbiology·Aaron LawThibault Stalder
Jul 1, 2021·Clinical Microbiology Reviews·F BaqueroT M Coque
Nov 19, 2021·Environmental Science & Technology·Johan Bengtsson-PalmeStefanie Heß

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