Abundance of endothelin-3 in rat intestine, pituitary gland and brain

Biochemical and Biophysical Research Communications
H MatsumotoM Fujino

Abstract

We established a highly sensitive and specific sandwich-enzyme immunoassay (EIA) for endothelin-3 (ET-3), which showed no crossreactivity with endothelin-1 (ET-1), endothelin-2 (ET-2) and big-endothelin-1 (big-ET-1). We had previously established a sensitive sandwich-EIA for ET-1, which fully crossreacted with ET-2, but not with ET-3 or big-ET-1. These EIAs were used to examine the tissue distribution of immunoreactive (ir-) ET-3 and compare them with those of ir-ET-1 (including ir-ET-2) in Sprague-Dawley rats. High concentrations of ir-ET-3 were found in the intestine, lung, pituitary gland and brain (greater than 100 pg/g wet tissue), ir-ET-1(ET-2) showed widespread distribution, with large amounts in the lung and colon (greater than 1000 pg/g wet tissue). The pituitary gland was the only organ containing higher amounts of ir-ET-3 than ir-ET-1 (ET-2). In reverse phase-high performance liquid chromatography coupled with EIAs, the ir-ET-3 was exclusively eluted at the position of synthetic ET-3, indicating that the ir-ET-3 was identical to ET-3. The abundance of ET-3 in the intestine, pituitary gland and brain indicates that ET-3 is a new brain-gut peptide which may have a physiological function in nervous and endocrine systems.

References

Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A InoueT Masaki
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M YanagisawaK Goto
Dec 2, 1983·Science·D T Krieger

❮ Previous
Next ❯

Citations

Jan 1, 1996·The Journal of Pathology·Y KakugawaW P Duguid
Jul 1, 1992·Medicinal Research Reviews·T MasakiK Goto
Jun 1, 1992·Pflügers Archiv : European journal of physiology·M RodenK Turnheim
Aug 1, 1995·Molecular and Cellular Biochemistry·S A RudgeC J Kirk
Aug 1, 1996·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·M BassiratZ Khalil
Apr 1, 1997·Journal of Gastroenterology·Y WatanabeY Kohgo
Nov 1, 1995·American Journal of Obstetrics and Gynecology·H OhbuchiK Toshimori
Jan 25, 1991·Brain Research·W K SamsonC E Gómez-Sánchez
May 30, 1991·European Journal of Pharmacology·Z WollbergE Kochva
Jun 6, 1991·European Journal of Pharmacology·T P BurrisM E Freeman
Dec 3, 1991·European Journal of Pharmacology·T NakamuraS Shimoda
Sep 4, 1992·European Journal of Pharmacology·W W Lin, C Y Lee
Jun 11, 1993·European Journal of Pharmacology·S RebelloA Gulati
Jan 1, 1990·Life Sciences·Y ShiboutaK Nishikawa
Jan 1, 1992·Life Sciences·T KuriharaY Hirayama
May 1, 1993·Toxicon : Official Journal of the International Society on Toxinology·E KochvaZ Wollberg
Nov 1, 1991·Journal of the Autonomic Nervous System·T KuwakiM Kumada
Mar 1, 1990·Trends in Pharmacological Sciences·W G Nayler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.