Abundances of transcripts, proteins, and metabolites in the cell cycle of budding yeast reveal coordinate control of lipid metabolism

Molecular Biology of the Cell
Heidi M BlankMichael Polymenis

Abstract

Establishing the pattern of abundance of molecules of interest during cell division has been a long-standing goal of cell cycle studies. Here, for the first time in any system, we present experiment-matched datasets of the levels of RNAs, proteins, metabolites, and lipids from unarrested, growing, and synchronously dividing yeast cells. Overall, transcript and protein levels were correlated, but specific processes that appeared to change at the RNA level (e.g., ribosome biogenesis) did not do so at the protein level, and vice versa. We also found no significant changes in codon usage or the ribosome content during the cell cycle. We describe an unexpected mitotic peak in the abundance of ergosterol and thiamine biosynthesis enzymes. Although the levels of several metabolites changed in the cell cycle, by far the most significant changes were in the lipid repertoire, with phospholipids and triglycerides peaking strongly late in the cell cycle. Our findings provide an integrated view of the abundance of biomolecules in the eukaryotic cell cycle and point to a coordinate mitotic control of lipid metabolism.

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Citations

May 8, 2021·Frontiers in Cell and Developmental Biology·Yongqiang LiuShan Gao
May 17, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Milada VítováSamuel Furse

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Datasets Mentioned

BETA
GSE135476

Methods Mentioned

BETA
RNAseq
flow cytometry
tandem affinity purification
reverse phase chromatography
chip

Key Resources (RRID) Mentioned

SCR_003093
SCR_015539
SCR_014329
SCR_003070
SCR_000432
SCR_004694
SCR_001905
SCR_002811

Software Mentioned

PANTHER
Biognosys
latticeExtra R language package
WRS2 R language
DESeq2
Rsubread
DESeq2 R language package
pheatmap R
RNAseq
Spectronaut

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