Abundant expression and hemimethylation of C19MC in cell cultures from placenta-derived stromal cells

Biochemical and Biophysical Research Communications
Inga FlorJörn Bullerdiek

Abstract

MicroRNAs of the chromosome 19 microRNA cluster (C19MC) are known to be abundantly expressed in the placenta. Their genes are located on the long arm of chromosome 19 and seem to be part of a large imprinted region. Although the data available so far suggest important functions in the placenta, no data are available on their general expression patterns in cultures of placenta-derived mesenchymal stromal cells (PDMSC). Surprisingly, qRT-PCR on tissue cultures from first-trimester and term placenta mesenchymal stromal cells showed an abundant expression of the cluster members miR-517a-3p, miR-519a-3p, and miR-520c-3p. Accordingly, analyses of methylation patterns suggested that these cells had escaped methylation and epigenetic silencing, respectively, of the paternal allele. This was confirmed by the results of treatment of chorionic villous stromal cells by the demethylating agent 5-Aza-2'-deoxycytidine. Our results offer clear evidence that, in contrast to what is suggested in previous papers, members of C19MC are highly expressed in PDMSC indicating that their placenta-specific functions are not restricted to the trophoblast.

References

Apr 1, 1992·American Journal of Medical Genetics·G S SongsterS W Cheung
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 21, 2005·Nature Genetics·Isaac BentwichZvi Bentwich
Feb 10, 2006·Genome Research·P V Krishna PantKelly A Frazer
Mar 9, 2006·American Journal of Obstetrics and Gynecology·C Bettina Portmann-LanzDaniel V Surbek
Jan 15, 2008·Nature Cell Biology·Qihong HuangReuven Agami
Apr 18, 2008·Molecular Biology and Evolution·Rui ZhangBing Su
May 20, 2008·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Robert PijnenborgMyriam Hanssens
Dec 17, 2008·British Journal of Haematology·Gary BrookeKerry Atkinson
Mar 25, 2009·Journal of Translational Medicine·Jiaqiang RenDavid F Stroncek
Nov 20, 2009·Epigenetics : Official Journal of the DNA Methylation Society·Kuo-Wang TsaiWen-chang Lin
Jul 9, 2010·Human Molecular Genetics·Marie Noguer-DanceJérôme Cavaillé
Mar 23, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·Jean-Francois MouilletYoel Sadovsky
Mar 3, 2012·Molecular Human Reproduction·R B DonkerYoel Sadovsky

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Citations

Jun 24, 2014·Cellular & Molecular Immunology·Mallikarjun BidarimathChandrakant Tayade
Feb 28, 2013·American Journal of Physiology. Endocrinology and Metabolism·Yang GuYuping Wang
Feb 26, 2013·Journal of Reproductive Immunology·Diana M Morales-PrietoUdo R Markert
Oct 2, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Sam BuckberryClaire T Roberts
Sep 24, 2016·JCI Insight·Graham M StrubJonathan A Perkins
Apr 12, 2013·Biology of Reproduction·Dong-bao Chen, Wen Wang
Feb 5, 2019·Frontiers in Genetics·E Cécile MalnouJérôme Cavaillé
Jul 18, 2017·Journal of Pregnancy·Meng CaiAsif Ahmed
Sep 27, 2018·Journal of Cellular Physiology·Yan LvMingming Lv

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