Acacetin Alleviates Inflammation and Matrix Degradation in Nucleus Pulposus Cells and Ameliorates Intervertebral Disc Degeneration in vivo.

Drug Design, Development and Therapy
Hao WangYutong Gu

Abstract

Intervertebral disc degeneration (IDD) is one of the most prevalent musculoskeletal disorders. The nucleus pulposus is the major component of the intervertebral disc, and nucleus pulposus cells (NPCs) play a significant role in the normal functioning of the intervertebral disc. Reactive oxygen species (ROS) generation, inflammation and extracellular matrix degradation in NPCs contribute to the degeneration of intervertebral discs. Acacetin is a drug that exerts antioxidant and anti-inflammatory effects on many types of cells. However, whether acacetin can relieve the degeneration of NPCs remains unknown. NPCs were extracted from rat intervertebral discs. The NPCs were treated with tert-butyl peroxide (TBHP) to simulate a high-ROS environment, and acacetin was subsequently added. The contents of ROS, inflammatory mediators (COX-2, iNOS) and extracellular matrix components (aggrecan, collagen II, MMP13, MMP9, MMP3) were measured. Components of related signaling pathways (Nrf2, MAPK) were also evaluated. To determine the effect of acacetin in vivo, we simulated disc degeneration via needle puncture. Acacetin was then applied intraperitoneally, and the degenerative status was evaluated using MRI and histopathological analysis. In v...Continue Reading

References

Oct 13, 2009·Joint, Bone, Spine : Revue Du Rhumatisme·Johann ClouetJérôme Guicheux
Oct 30, 2013·Nature Reviews. Rheumatology·Makarand V Risbud, Irving M Shapiro
Oct 1, 2015·The Journal of Nutritional Biochemistry·Ying HuangAh-Ng Tony Kong
Oct 18, 2016·Lancet·Chris MaherRachelle Buchbinder
Apr 11, 2017·Oxidative Medicine and Cellular Longevity·Chencheng FengYue Zhou
Aug 9, 2017·Scientific Reports·Pengmian FengWei Chen
Nov 2, 2017·Bioorganic & Medicinal Chemistry Letters·Sang Min KimSeung-Hwan Kwon
Feb 3, 2018·Oxidative Medicine and Cellular Longevity·Congcong FangXiaoxing Xiong
Oct 3, 2018·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Xudong YaoFengjing Guo
Jun 24, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Wen-Ze XiaoXin Zhao
Nov 2, 2019·Ageing Research Reviews·Sidong YangWenyuan Ding
Feb 19, 2020·Journal of Cellular and Molecular Medicine·Ping ShangHaixiao Liu
Mar 4, 2020·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Eun-Bin KwonMun-Ock Kim
Mar 7, 2020·International Journal of Molecular Sciences·Carla CunhaRaquel M Goncalves
May 14, 2020·World Journal of Stem Cells·Yang LiuLiang Zhang
May 24, 2020·International Journal of Molecular Sciences·Petra Cazzanelli, Karin Wuertz-Kozak
Jul 1, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Veerta SharmaThakur Gurjeet Singh

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Methods Mentioned

BETA
Assay
electrophoresis
PCR
flow cytometry
ubiquitination

Software Mentioned

GraphPad
GraphPad Prism

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