Acanthamoeba castellanii: structural basis of the cytopathic mechanisms

Experimental Parasitology
Arturo González-RoblesAdolfo Martínez-Palomo

Abstract

In this study we report observations on the structural mechanisms of the cytopathic effect of Acanthamoeba castellanii trophozoites on cultured MDCK cell monolayers. Co-incubations were carried out for a maximum of 24h. The first evidence of damage to the cell monolayer was detected by measuring the transepithelial resistance of cell monolayers that interacted with the amoebae. At 6h, transepithelial resistance diminished to 51% and amoebae required 5-6h to produce evidence of structural injury at the light microscopy level. Following 12h of incubation, the cell monolayer was severely damaged. After making intimate contact with the surface of target cells, trophozoites detached cells from the substrate, lysed and by means of food-cups ingested the damaged cells. There was no morphological evidence of modifications in MDCK cell membranes, membrane fusion or junction formation between the amoeba and host plasma membrane. The lytic capacity of the amoebas appears to be the result of cytotoxic factors secreted by the amoebae since, when monolayers were incubated with conditioned medium, there was also a decrease in the transepithelial resistance. Besides, mechanical injury produced by the attachment and movement of the trophozoites...Continue Reading

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Citations

Feb 12, 2010·Experimental Parasitology·Maritza Omaña-MolinaAdolfo Martínez-Palomo
Oct 14, 2008·International Journal for Parasitology·Fernando López-SotoMireya de la Garza
Dec 16, 2006·The Journal of Eukaryotic Microbiology·Maritza Omaña-MolinaPatricia Bonilla
Apr 4, 2015·Protist·Luiz Carlos RibeiroMarlene Benchimol
Oct 15, 2014·Journal of Parasitology Research·Arturo González-RoblesJacob Lorenzo-Morales
Nov 2, 2019·Parasitology International·Daniel Coronado-VelázquezJesús Serrano-Luna

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