Abstract
The plant hormone ethylene has been reported to inhibit the Agrobacterium tumefaciens-mediated transformation efficiency of many plants. In this study, an acdS gene that encodes 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, an enzyme that breaks down ACC, the direct precursor of ethylene biosynthesis in all higher plants, was introduced into A. tumefaciens GV3101::pMP90. It was found that the presence of active ACC deaminase in A. tumefaciens reduced ethylene levels produced by plant tissues during the process of infection and cocultivation, and significantly increased the transformation efficiency of three commercial canola cultivars: Brassica napus cv. Westar, B. napus cv. Hyola 401 and B. napus cv. 4414RR.
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Citations
Sep 4, 2013·MicrobiologyOpen·Tatsuhiko SomeyaHiroshi Ezura
Dec 19, 2014·Frontiers in Plant Science·Satoko Nonaka, Hiroshi Ezura
Feb 22, 2017·Scientific Reports·Satoko NonakaHiroshi Ezura
Mar 10, 2018·Frontiers in Plant Science·Francisco X NascimentoBernard R Glick
Oct 28, 2019·Frontiers in Plant Science·Satoko NonakaHiroshi Ezura
Nov 3, 2020·Frontiers in Plant Science·Uyen Cao ChuTodd J Jones