Accelerate Healing of Severe Burn Wounds by Mouse Bone Marrow Mesenchymal Stem Cell-Seeded Biodegradable Hydrogel Scaffold Synthesized from Arginine-Based Poly(ester amide) and Chitosan

Stem Cells and Development
Bhagwat V AlapureSong Hong

Abstract

Severe burns are some of the most challenging problems in clinics and still lack ideal modalities. Mesenchymal stem cells (MSCs) incorporated with biomaterial coverage of burn wounds may offer a viable solution. In this report, we seeded MSCs to a biodegradable hybrid hydrogel, namely ACgel, that was synthesized from unsaturated arginine-based poly(ester amide) (UArg-PEA) and chitosan derivative. MSC adhered to ACgels. ACgels maintained a high viability of MSCs in culture for 6 days. MSC seeded to ACgels presented well in third-degree burn wounds of mice at 8 days postburn (dpb) after the necrotic full-thickness skin of burn wounds was debrided and filled and covered by MSC-carrying ACgels. MSC-seeded ACgels promoted the closure, reepithelialization, granulation tissue formation, and vascularization of the burn wounds. ACgels alone can also promote vascularization but less effectively compared with MSC-seeded ACgels. The actions of MSC-seeded ACgels or ACgels alone involve the induction of reparative, anti-inflammatory interleukin-10, and M2-like macrophages, as well as the reduction of inflammatory cytokine TNFα and M1-like macrophages at the late inflammatory phase of burn wound healing, which provided the mechanistic insight...Continue Reading

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Citations

Jul 17, 2019·Journal of Tissue Engineering and Regenerative Medicine·Timo RademakersVanessa L S LaPointe
May 20, 2020·Advanced Healthcare Materials·Fan Zhang, Martin W King
Feb 16, 2021·International Journal of Nanomedicine·Carolina Maria Costa de Oliveira SouzaKatherine Athayde Teixeira de Carvalho
Feb 18, 2021·Carbohydrate Polymers·Sara TorkamanSeyed Heydar Mahmoudi Najafi
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Mar 3, 2021·International Journal of Biological Macromolecules·Jose Luis Sanchez-SalvadorAngeles Blanco
Mar 7, 2021·International Journal of Molecular Sciences·Hantae JoBum-Ho Bin
Mar 24, 2021·Tissue & Cell·Sana UllahMuhammad Ali
Jul 3, 2021·Burns and Trauma·Mingyao WangHuiqi Xie
Jul 29, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Parisa HeydariShaghayegh Haghjooy Javanmard
Aug 5, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yang ZhouJun Wu
Aug 8, 2021·Cells·Raquel Guillamat-Prats

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