Accelerated exchange of a buried water molecule in selectively disulfide-reduced bovine pancreatic trypsin inhibitor

Biochemistry
V P DenisovBertil Halle

Abstract

Using magnetic relaxation dispersion (MRD), we have previously shown that the four internal water molecules in bovine pancreatic trypsin inhibitor (BPTI) exchange with bulk water on time scales between 10(-8) and 10(-4) s at room temperature. Because this exchange is controlled by the protein structure, internal water molecules can be used to probe rare conformational fluctuations. Here, we report (2)H and (17)O MRD data at three temperatures for wild-type BPTI and two BPTI variants where the 14-38 disulfide bond has been cleaved by a double Cys --> Ser mutation or by disulfide reduction and carboxamidomethylation. The MRD data show that the internal water molecules are conserved on disulfide cleavage. However, the exchange rate of the water molecule buried near the disulfide bond is enhanced by 2-4 orders of magnitude. The relation of water exchange to other dynamic processes in BPTI is discussed.

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Citations

May 17, 2013·Journal of the American Chemical Society·Filip Persson, Bertil Halle
Sep 22, 2011·The Journal of Physical Chemistry. B·Jean-Pierre KorbRobert G Bryant
Jun 10, 2018·The Journal of Chemical Physics·Filip PerssonBertil Halle
Jan 11, 2008·Journal of the American Chemical Society·Erik Persson, Bertil Halle

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