Accelerated extraction for determination of polycyclic aromatic hydrocarbons in marine biota

Analytical and Bioanalytical Chemistry
J Sanz-LandaluzeC Cámara

Abstract

A rapid and simple method is proposed for determination of polycyclic aromatic hydrocarbons (PAH) in complex matrices such as marine biota. The method uses sonication, by means of an ultrasonic probe, as a new tool for assisted extraction, coupled with reversed-phase liquid chromatography (RP-LC) with fluorescence detection (FL) for determination of 16 US EPA priority PAH. Separation and detection of the 16 PAH were complete in 45 min by RP-LC with a C18 column and acetonitrile-water gradient elution. Multivariate optimisation of the variables affecting extraction (ultrasound radiation amplitude, sonication time, and temperature of the water-bath in which the extraction cell was placed) was conducted. The accuracy of the method was determined by analysis of a certified reference material and comparison of the results obtained with those from another method (microwave-assisted extraction and GC-MS). The new technique avoids the main problems encountered in the determination of PAH in complex matrices such as marine biota, and no clean-up step is necessary. The method was applied to determination of PAH in estuarine biota samples from the Urdaibai estuary (Biscay, Spain).

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Citations

Apr 21, 2009·Analytical and Bioanalytical Chemistry·Tuulia Hyötyläinen
Apr 25, 2012·Analytical and Bioanalytical Chemistry·María Pilar Martínez-Moral, María Teresa Tena
Feb 23, 2012·Environmental Science & Technology·Ioannis SadiktsisRoger Westerholm
Dec 28, 2010·Journal of Chromatography. a·Cristina Moreta, María Teresa Tena
Apr 27, 2010·Journal of Chromatography. a·J Sanz-LandaluzeC Cámara
Sep 15, 2009·Journal of Separation Science·Maria João RamalhosaMaria Beatriz Prior Pinto Oliveira
Dec 1, 2012·The Science of the Total Environment·Eunate Puy-AzurmendiMiren P Cajaraville
Dec 22, 2009·Journal of Separation Science·Asier VallejoLuis A Fernández

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