Accelerated insulin aggregation under alternating current electric fields: Relevance to amyloid kinetics

Biomicrofluidics
Zhongli ZhengYingxi Zhu

Abstract

The time-dependent nucleation phase is critical to amyloid fibrillation and related to many pathologies, in which the conversion from natively folded amyloidogenic proteins to oligomers via nucleation is often hypothesized as a possible underlying mechanism. In this work, non-uniform AC-electric fields across two asymmetric electrodes were explored to control and examine the aggregation of insulin, a model amyloid protein, in aqueous buffer solution at constant temperature (20 °C) by fluorescence correlation spectroscopy and fluorescence microscopy. Insulin was rapidly concentrated in a strong AC-field by imposed AC-electroosmosis flow over an optimal frequency range of 0.5-2 kHz. In the presence of an AC-field, direct fibrillation from insulin monomers without the formation of oligomer precursors was observed. Once the insulin concentration had nearly doubled its initial concentration, insulin aggregates were observed in solution. The measured lag time for the onset of insulin aggregation, determined from the abrupt reduction in insulin concentration in solution, was significantly shortened from months or years in the absence of AC-fields to 1 min-3 h under AC-fields. The ability of external fields to alter amyloid nucleation ...Continue Reading

References

Oct 1, 1990·International Journal of Peptide and Protein Research·M DatheB Mehlis
Mar 1, 1987·Diabetes·E Helmerhorst, G B Stokes
May 25, 1995·Nucleic Acids Research·M Kinjo, R Rigler
Feb 19, 2000·Current Opinion in Structural Biology·J C Rochet, P T Lansbury
Nov 23, 2000·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·N G GreenA Castellanos
Jul 18, 2002·Nature Reviews. Drug Discovery·James C Sacchettini, Jeffery W Kelly
Dec 20, 2003·Nature·Christopher M Dobson
Jul 24, 2004·Nature Medicine·Christopher A Ross, Michelle A Poirier
Aug 6, 2004·Protein Science : a Publication of the Protein Society·Anubhav AroraChan Beum Park
Oct 19, 2004·Biophysical Journal·Luben N Arnaudov, Renko de Vries
Sep 17, 2005·Nature Reviews. Molecular Cell Biology·Christopher A Ross, Michelle A Poirier
Nov 23, 2005·PLoS Biology·Douglas M FowlerJeffery W Kelly
Dec 3, 2005·Protein Science : a Publication of the Protein Society·Fabio Librizzi, Christian Rischel
Jun 8, 2006·Annual Review of Biochemistry·Fabrizio Chiti, Christopher M Dobson
Jul 1, 2006·Nature·Motomasa TanakaJonathan S Weissman
Feb 28, 2007·Biophysical Journal·Chuang-Chung LeeGregory J McRae
Apr 7, 2007·Trends in Biochemical Sciences·Douglas M FowlerJeffery W Kelly
Jun 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tuomas P J KnowlesMark E Welland
Jul 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Amy M Ruschak, Andrew D Miranker
Nov 1, 2007·Nature Methods·Sally A KimPetra Schwille
May 9, 2008·Journal of Molecular Biology·Alex Perálvarez-MarínAstrid Gräslund
Jun 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Wei-Feng XueSheena E Radford
Jul 26, 2008·Proteins·Arpan NayakGeorges Belfort
Dec 17, 2008·Biochimica Et Biophysica Acta·Aimee M MorrisRichard G Finke
May 6, 2009·Biophysical Journal·Shannon E HillMartin Muschol
Oct 30, 2009·Proceedings of the National Academy of Sciences of the United States of America·Magdalena I IvanovaDavid Eisenberg
Dec 17, 2009·Science·Tuomas P J KnowlesChristopher M Dobson
Mar 26, 2010·Journal of the American Chemical Society·Duncan A WhiteChristopher M Dobson
Aug 19, 2010·Biotechnology and Bioengineering·Caryn L HeldtGeorges Belfort
Dec 16, 2010·Biophysical Journal·Leonard F PeaseGeorges Belfort
May 3, 2011·Journal of the American Chemical Society·Alexander K BuellChristopher M Dobson
Dec 20, 2011·Colloids and Surfaces. B, Biointerfaces·Victor Banerjee, K P Das
Apr 21, 2012·Science·David Eliezer
Dec 3, 2014·Journal of Molecular Biology·Frank A Ferrone
Mar 25, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Brian MurrayGeorges Belfort

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Feb 3, 2018·The Journal of Chemical Physics·Yan LuPhilippe Derreumaux
Jul 25, 2019·Physical Review. E·S M H Hashemi AmreiWilliam D Ristenpart
Sep 16, 2020·Biophysics Reviews·Gaurav Pandey, Vibin Ramakrishnan
Apr 17, 2017·The Journal of Chemical Physics·Yan LuPhilippe Derreumaux
Feb 2, 2019·ACS Chemical Neuroscience·Jahnu SaikiaVibin Ramakrishnan

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