Accelerated Mineralization of Pentachlorophenol in Soil upon Inoculation with Mycobacterium chlorophenolicum PCP1 and Sphingomonas chlorophenolica RA2.

Applied and Environmental Microbiology
R Miethling, U Karlson

Abstract

Mineralization of pentachlorophenol (PCP) was studied in nonsterile soil from a PCP-contaminated site upon inoculation with two PCP-degrading bacterial strains. At spiked [(sup14)C]PCP concentrations of 30 and 100 mg/kg, the effects of organism type, different inoculation techniques, including structural amendment with sawdust and cell attachment to polyurethane (PU), as well as the effect of different inoculum sizes of 10(sup4) to 10(sup8) cells per g (dry weight) of soil were compared with PCP mineralization by indigenous bacteria. Gas chromatographic analysis was used to monitor PCP disappearance and to check mass balances. The survival and activity of the released bacteria were examined by immunofluorescence microscopy and respiking experiments. Noninoculated soil completely mineralized 30 mg of PCP per kg within 7 months but showed no or only low degradation activity at 100 mg/kg in the same period. Structural amendment with PU or sawdust initiated slow mineralization after half a year. Soil inoculation with Sphingomonas chlorophenolica RA2 shortened the mineralization time drastically to 1 month at 30 mg of PCP per kg using 10(sup8) cells per g, with approximately 80% of the added radioactivity being converted to CO(inf2)...Continue Reading

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Citations

Nov 13, 2008·Applied Microbiology and Biotechnology·Andreas Stolz
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Shinawar Waseem AliShun-peng Li
May 16, 2002·Journal of Applied Microbiology·L CavalcaV Andreoni
Jan 31, 2009·FEMS Microbiology Reviews·Janmejay PandeyRakesh K Jain
May 27, 2008·Bulletin of Environmental Contamination and Toxicology·Ashwani Sharma, Indu Shekhar Thakur
Dec 10, 2003·The Journal of Biological Chemistry·Luying Xun, Chris M Webster
Apr 25, 2001·Applied and Environmental Microbiology·P Y Cheung, B K Kinkle
Jun 1, 1997·Microbiology and Molecular Biology Reviews : MMBR·J A van VeenJ D van Elsas

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