Accelerated recombinant protein purification process development automated, robotics-based integration of chromatographic purification and analysis

Journal of Chromatography. a
T LondoN Gordon

Abstract

Recovery of recombinant proteins from endogenous, host molecules can be an experimentally intensive and time-consuming task. Often the time to analyze material during development of recovery procedures is the rate-limiting step. Nowadays, modern techniques and equipment are being specifically engineered to make this effort much more efficient. We present a case study to illustrate how a new, automation tool, designed for easy, systematic methods development, can be used for very rapid process and analytical optimization. This tool uses robotics to integrate process development with rapid LC-based analysis requiring no user intervention. The methods and procedures described can be generalized to any recombinant protein recovery campaign.

References

Dec 1, 1993·Analytical Chemistry·S K PaliwalF E Regnier
Feb 1, 1996·Analytical Chemistry·Y L HsiehF Regnier

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Citations

Oct 10, 2009·Trends in Biotechnology·Beckley K NforMarcel Ottens
Dec 22, 2005·Biotechnology and Bioengineering·Kaushal RegeMeng Heng

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