Acceleration of apoptosis in CD4+CD8+ thymocytes by rapamycin accompanied by increased CD4+CD25+ T cells in the periphery

Transplantation
Lina TianPaul K H Tam

Abstract

Rapamycin (Rapa) is an immunosuppressant that is used in patients and animal models to control allograft rejection. Its mechanisms of action are not fully understood. In this article, the authors have investigated the effects of therapeutic doses of Rapa on both thymic and peripheral T-cell populations in the adult rat. The therapeutic dosage of Rapa was optimized using cardiac transplantation between LEW and DA rats. Thymic morphology was assessed by hematoxylin-eosin staining. Flow cytometric analysis was performed to analyze T-cell phenotype and apoptosis. T-cell receptor (TCR)-mediated T-cell responsiveness was evaluated by 3[H]-thymidine deoxyribose incorporation. Rapa induced atrophy in the thymus but not in peripheral lymphoid organs. Moreover, fibrosis occurred in thymus that was long-lasting after Rapa withdrawal. In animals treated with Rapa, there was a significant reduction in CD4+CD8+ thymocytes caused by accelerated apoptosis, whereas CD4-CD8-, CD4+CD8-, and CD8+CD4- populations remained unaffected. In contrast, the cellularity of the periphery lymphoid organs was not altered. Within the CD4+ thymocyte population, CD4+CD25+ thymocytes were resistant to Rapa-accelerated apoptosis, and in the periphery, the ratio of...Continue Reading

References

Sep 23, 1988·Science·M K JenkinsD M Pardoll
Mar 1, 1994·European Journal of Immunology·H LuoJ Wu
Jan 1, 1996·Annual Review of Immunology·R T Abraham, G J Wiederrecht
Aug 10, 2000·Immunological Reviews·L Lu, D G Osmond
Nov 9, 2000·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·B J ChenN J Chao
Jan 3, 2001·Kidney International·R N SaundersM L Nicholson
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M HaraK J Wood
Oct 17, 2001·Transplantation·B D Kahan, J S Camardo
Jan 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cherry I KingsleyKathryn J Wood
Jul 13, 2002·European Journal of Immunology·Leonie S TaamsArne N Akbar

❮ Previous
Next ❯

Citations

Jun 25, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Svetlana StanojlovicUwe Pleyer
Aug 22, 2007·Cell Biochemistry and Biophysics·Alex Rabinovitch, Wilma L Suarez-Pinzon
Jun 16, 2005·Current Opinion in Pharmacology·Deborah A Young, Cheryl L Nickerson-Nutter
Oct 22, 2005·Nature Reviews. Immunology·Casey J FoxCraig B Thompson
Aug 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rishu GuoXiao-Ping Zhong
Jan 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Wei GuoHong Wu
Jan 12, 2005·The Journal of Experimental Medicine·Casey J FoxCraig B Thompson
Jun 24, 2008·BMC Cancer·Elena V SvirshchevskayaLyuba Varticovski
Oct 11, 2013·Kidney International·Giovanni StalloneLoreto Gesualdo
Sep 17, 2011·Seminars in Immunology·Huimin FanShuiping Jiang
May 14, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Elena DevyatkoGuenter Weigel
Feb 19, 2008·Journal of Pediatric Surgery·Paul K H Tam
Feb 15, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·David S GameRobert I Lechler
May 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Gregory I SnellTrevor J Williams
Dec 22, 2006·Transplant International : Official Journal of the European Society for Organ Transplantation·Dela GolshayanManuel Pascual
Aug 30, 2008·The Mount Sinai Journal of Medicine, New York·Jennifer BollykyCarla J Greenbaum
Sep 2, 2008·Immunological Reviews·Xiao-Ping ZhongChi-Keung Wan
Oct 5, 2006·Transplant International : Official Journal of the European Society for Organ Transplantation·Dela Golshayan, Manuel Pascual
Aug 19, 2007·Journal of Internal Medicine·M Sykes
Aug 26, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J M Grinyó, J M Cruzado
Jan 8, 2011·Transplant International : Official Journal of the European Society for Organ Transplantation·Orsola PuglieseFrancesca Quintieri
Apr 12, 2013·Clinical and Experimental Immunology·N SafiniaG Lombardi
Oct 8, 2009·International Journal of Cancer. Journal International Du Cancer·Marta López-FauquedJuan A Recio
Sep 3, 2004·Current Opinion in Immunology·Shuiping JiangRobert I Lechler
Dec 13, 2006·International Immunopharmacology·Julia TsangRobert I Lechler
Jan 25, 2006·Seminars in Cancer Biology·Emmanuel Zorn
Nov 7, 2006·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Vu H NguyenRobert S Negrin
Apr 21, 2009·Pharmacology & Therapeutics·Gopala K RanganKok On Ho
Aug 2, 2016·Nature Reviews. Nephrology·Daniel FantusAngus W Thomson
Aug 31, 2007·Clinical Pharmacology and Therapeutics·C M Hartford, M J Ratain

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.