PMID: 8444193Mar 1, 1993Paper

Acceleration of bovine neurofilament L assembly by deprivation of acidic tail domain

European Journal of Biochemistry
Y NakamuraT Nishimura

Abstract

Functions of the tail region of neurofilament L have, to date, not been clearly elucidated. Bovine neurofilament L was cleaved into tail-less neurofilament L (50 kDa) and a tail fragment (19 kDa), by thrombin. Tail-less neurofilament L was deficit of the highly acidic domain of the tail region (approximately 77% of the entire region). Assembly of tail-less neurofilament L. was observed to be accelerated by both fluorometric and centrifugal measurements, compared with intact neurofilament L. The critical concentration of tail-less neurofilament L, which constitutes the constant unassembled pool, was approximately 0.25-times lower than that of neurofilament L. Under physiological conditions, tail-less neurofilament L formed a ribbon-like structure, whereas tail-less neurofilament L could form 10-nm filaments in an extremely low ionic-strength buffer in the presence of 1 mM MgCl2. An affinity-purified antibody directed against the tail fragment also accelerated neurofilament L assembly. The tail fragment neither coassembled with neurofilament L nor affect neurofilament L assembly. The acidic domain of the tail region may regulate neurofilament assembly and may be involved in 10-nm filament formation under physiological conditions.

References

Jul 1, 1992·The Journal of Cell Biology·K L VikstromG G Borisy
Jun 15, 1990·Biochemical and Biophysical Research Communications·Y NakamuraT Nishimura
Feb 23, 1990·Cell·P M Steinert, R K Liem
Sep 1, 1985·Cell·P M SteinertD R Roop
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K L VikstromR D Goldman
Aug 15, 1987·The Biochemical Journal·M H StromerR M Robson

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Citations

Sep 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R L Shoeman, P Traub
Apr 11, 1997·Neuroscience Letters·Y NakamuraM Takeda
Feb 1, 1994·Current Opinion in Cell Biology·S Heins, U Aebi
Feb 7, 2020·Scientific Reports·Benjamin L SpringsteinKarina Stucken

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