Abstract
The purpose of our study was to investigate the effect of adipose-derived stem cells (ASCs), endothelial-differentiated ASCs (EC/ASCs), and various conditioned media (CM) on wound healing in a diabetic swine model. We hypothesized that ASC-based therapies would accelerate wound healing. Diabetes was induced in four Yorkshire swine through intravenous injection of streptozotocin. ASCs were harvested from flank fat and cultured in either M199 or EGM-2 medium. A duplicate series of seven full-thickness dorsal wounds were surgically created on each swine. The wounds in the cellular treatment group underwent injection of low-dose or high-dose ASCs or EC/ASCs on day 0, with a repeat injection of one half of the initial dose on day 15. Wounds assigned to the topical CM therapy were covered with 2 mL of either serum-free M199 primed by ASCs or human umbilical vein endothelial cells every 3 days. Wounds were assessed at day 0, 10, 15, 20, and 28. The swine were sacrificed on day 28. ImageJ software was used to evaluate the percentage of wound healing. The wounded skin underwent histologic, reverse transcription polymerase chain reaction, and enzyme-linked immunosorbent assay examinations to evaluate markers of angiogenesis and inflammat...Continue Reading
Citations
Dec 12, 2019·Journal of Tissue Engineering and Regenerative Medicine·Aristotelis KotronoulasÓttar Rolfsson
Oct 14, 2019·International Wound Journal·Evi M MorandiRalph Verstappen
Apr 28, 2020·Current Eye Research·Sayena JabbehdariAli R Djalilian
Jul 30, 2020·Angiology·Francisco Javier Álvaro-AfonsoNikolaos Papanas
Sep 24, 2020·Advances in Wound Care·Xin TangWei Li
Sep 29, 2019·Journal of Diabetes Research·Cheng ZhangYimin Chai
Aug 2, 2019·International Journal of Molecular Sciences·Francesca LombardiMaurizio Giuliani
Dec 6, 2018·Journal of Cellular Physiology·Alireza HassanshahiCory J Xian
Oct 16, 2019·Stem Cells Translational Medicine·Karlien KallmeyerAli Modarressi
Feb 6, 2020·Stem Cell Research & Therapy·Mingwu DengWen Jie Zhang
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Yiyao ZhangXifan Mei
Jul 6, 2020·Trends in Biotechnology·Leila DaneshmandiCato T Laurencin
Feb 27, 2020·BMJ Open Diabetes Research & Care·Roohollah Ebrahimpour-MalekshahMohammad Bayat
Apr 13, 2021·Bioscience Reports·Lulu WangLei Shen
May 22, 2021·Plastic and Reconstructive Surgery·Hiroko TakahashiEmi Funayama
May 25, 2021·Aging·Mei-Hua JinYing-Hao Han
Jul 15, 2021·Stem Cell Research & Therapy·Nesrine EbrahimRabab F Salim
Aug 10, 2021·Frontiers in Cell and Developmental Biology·Trinidad Montero-VilchezSalvador Arias-Santiago
Jul 25, 2018·ACS Nano·Weizong WengJiacan Su
Aug 20, 2021·Tissue Engineering. Part B, Reviews·Bihang ZhangKotaro Yoshimura
Feb 21, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Satish PatelDeependra Singh
Oct 4, 2021·Journal of Nanobiotechnology·Huiling SunXinwang Yang