PMID: 8997665Jan 1, 1997Paper

Acceleration of sodium-calcium exchange activity during ATP-induced calcium release in transfected Chinese hamster ovary cells

The Journal of General Physiology
M VázquezJ P Reeves

Abstract

The P2U purinergic agonist ATP (0.3 mM) elicited an increase in [Ca2+]i due to Ca2+ release from intracellular stores in transfected Chinese hamster ovary cells that express the bovine cardiac Na+/Ca2+ exchanger (CK1.4 cells). The following observations indicate that ATP-evoked Ca2+ release was accompanied by a Ca(2+)-dependent regulatory activation of Na+/Ca2+ exchange activity: Addition of extracellular Ca2+ (0.7 mM) 0-1 min after ATP evoked a dramatic rise in [Ca2+]i in Na(+)-free media (Li+ substitution) compared to Na(+)-containing media; no differences between Na(+)- and Li(+)-based media were observed with vector-transfected cells. In the presence of physiological concentrations of extracellular Na+ and Ca2+, the ATP-evoked rise in [Ca2+]i declined more rapidly in CK1.4 cells compared to control cells, but then attained a long-lived plateau of elevated [Ca2+]i which eventually came to exceed the declining [Ca2+]i values in control cells. ATP elicited a transient acceleration of exchange-mediated Ba2+ influx, consistent with regulatory activation of the Na+/Ca2+ exchanger. The acceleration of Ba2+ influx was not observed in vector-transfected control cells, or in CK1.4 cells in the absence of intracellular Na+ or when the...Continue Reading

References

Jan 1, 1992·Trends in Pharmacological Sciences·J Garcia-SanchoC Villalobos
Jan 11, 1990·Pharmacology & Therapeutics·J W Putney
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·A B Parekh, R Penner
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Apr 1, 1993·The American Journal of Physiology·V PijuanJ B Smith
Jun 7, 1996·The Journal of Biological Chemistry·T IwamotoM Shigekawa

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Citations

Jan 28, 1999·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Y FangL He
Oct 29, 2003·The Journal of General Physiology·John P Reeves, Madalina Condrescu

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