PMID: 3748274Jul 1, 1986Paper

Acceptors for cyclic AMP-dependent and calcium ion-dependent protein kinases in rat brain cytosol fractions: a comparison of occluded (synaptosomal) cytosol with non-occluded cytosol

Neurochemical Research
R RodnightA Dosemeci

Abstract

Endogenous protein phosphorylation patterns were compared in occluded and non-occluded cytosol fractions prepared from rat forebrain. The occluded fraction was taken as representative of synaptosomal cytosol. One- and two-dimensional autoradiographs revealed the presence in non-occluded cytosol of a substrate for cAMP- and Ca2+/calmodulin-dependent protein kinase activities of Mr 300kD, corresponding to phosphorylated microtubule-associated protein-2 (MAP-2); this protein was absent in occluded cytosol. In contrast, a major substrate for protein kinase C was observed exclusively in occluded cytosol after phosphorylation under basal conditions. However, after phosphorylation in the presence of exogenous lipids, approximately equal amounts of the 82kD substrate were detected in both fractions, suggesting that protein kinase C in the occluded fraction was present in a partially activated state. Other minor differences in phosphorylation patterns between the two fractions were observed.

References

Feb 9, 1979·Molecular and Cellular Biochemistry·Y NishizukaH Yamamura
Apr 1, 1985·Journal of Neuroscience Methods·R RodnightC Perrett
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K A AlbertP Greengard
Oct 13, 1983·Nature·E J Nestler, P Greengard
Oct 1, 1984·British Journal of Haematology·L A CasoriaS R Goodman
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L I BinderL I Rebhun
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J P O'CallaghanW Lovenberg
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W C WuP Greengard
Jan 1, 1982·Progress in Brain Research·H R MahlerR G Sorensen
Jan 1, 1981·Developmental Neuroscience·H Holmes, R Rodnight

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