PMID: 8990164Jan 7, 1997Paper

Accessibility of nuclear DNA to triplex-forming oligonucleotides: the integrated HIV-1 provirus as a target

Proceedings of the National Academy of Sciences of the United States of America
Carine GiovannangeliC Hélène

Abstract

The control of gene transcription by antigene oligonucleotides rests upon the specific recognition of double-helical DNA by triplex-forming oligonucleotides. The development of the antigene strategy requires access to the targeted DNA sequence within the chromatin structure of the cell nucleus. In this sudy we have used HIV-1 chronically infected cells containing the HIV provirus as endogenous genes to demonstrate that the integrated HIV-1 proviral genome is accessible to triplex-forming oligonucleotides within cell nuclei. An oligonucleotide-psoralen conjugate targeted to the polypurine tract (PPT) of the HIV-1 proviral sequence was used as a tool to convert the noncovalent triple-helical complex into a covalent lesion on genomic DNA after UV irradiation of cells. Triplex-derived adducts were analyzed using two different methods. The photo-induced psoralen cross-link prevented cleavage of the target sequence by DraI restriction endonuclease, and the sequence-specific inhibition of cleavage was revealed and quantitated by Southern blot analysis. A quantitative analysis of cross-linking efficiency was also carried out by a competitive PCR-based assay. These two approaches allowed us to demonstrate that a triplex-forming oligonuc...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C GiovannangéliC Hélène
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J P LeonettiB Lebleu
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·E H PostelM E Hogan
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M TakasugiC Hélène
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E VerdinA Burny
Nov 1, 1995·Nature Medicine·C A Stein
Jul 25, 1995·Nucleic Acids Research·J K ChenS M Gryaznov
Apr 6, 1995·Nature·A M KriegD M Klinman
Apr 21, 1995·The Journal of Biological Chemistry·E F MichelottiD Levens
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·T L BurgessT B Kramer
Mar 1, 1995·Molecular and Cellular Biology·G WangP M Glazer
Apr 8, 1994·Cell·A P Wolffe
Feb 25, 1993·Nucleic Acids Research·F S CeliA R Shuldiner
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M GrigorievA Harel-Bellan
Jan 1, 1996·Cancer Investigation·L J Maher
Mar 8, 1996·The Journal of Biological Chemistry·J S BishopN Chaudhary
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·A M GewirtzP M Glazer
Apr 15, 1996·Nucleic Acids Research·S GryaznovB Calabretta
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·C EscudéC Hélène
Jan 20, 1996·AIDS Research and Human Retroviruses·M ComarM Giacca

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Citations

Sep 1, 2001·The Journal of Gene Medicine·M Faria, C Giovannangeli
May 12, 2000·Biochimica Et Biophysica Acta·D PraseuthC Hélène
May 12, 2000·Biochimica Et Biophysica Acta·S M Gryaznov
May 24, 2003·Biochimie·Lionel GuittatJean-Louis Mergny
Feb 15, 2001·International Journal of Radiation Oncology, Biology, Physics·O A SedelnikovaR D Neumann
Apr 9, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·S Sasaki
Sep 12, 2007·Biochemistry·Zhaoyang YeRam I Mahato
Feb 9, 2013·Chemical Reviews·Osman DolucaVyacheslav V Filichev
Oct 2, 2002·Journal of Biomolecular Structure & Dynamics·Mrinal Kanti GhoshRamesh Chandra
May 21, 1998·Antisense & Nucleic Acid Drug Development·M Egli
Feb 24, 2001·Antisense & Nucleic Acid Drug Development·O A SedelnikovaR D Neumann
Mar 25, 2005·Oligonucleotides·Amy J ZiembaScot W Ebbinghaus
Dec 25, 2007·Oligonucleotides·Zhaoyang YeRam I Mahato
Sep 1, 1997·Nucleic Acids Research·E S BelousovR B Meyer
Mar 21, 1998·Nucleic Acids Research·K Pongracz, S M Gryaznov
Jan 11, 2000·Nucleic Acids Research·P B ArimondoC Hélène
May 10, 2002·Nucleic Acids Research·Rivka GoobesAbraham Minsky
Jun 18, 2005·Nucleic Acids Research·Jennifer M KalishPeter M Glazer
Aug 5, 2008·Nucleic Acids Research·Maria DucaPaola B Arimondo
Aug 21, 2010·Journal of Nucleic Acids·Rajagopal Krishnan, Dennis H Oh
Feb 14, 2009·International Journal of Medical Sciences·Waldemar WaldeckKlaus Braun
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·M FariaC Giovannangeli
Jul 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·Z LuoP M Glazer
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·C EscudéC Hélène
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·D H OhP C Hanawalt
Jan 25, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Young-Wook WonYong-Hee Kim
Mar 8, 2016·ACS Chemical Biology·Frederic GeinguenaudLaurence Motte
Aug 27, 2004·Journal of Molecular Biology·Robert BeschKlaus Degitz

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