Accessing the inaccessible: molecular tools for bifidobacteria.

Applied and Environmental Microbiology
Zhongke SunChristian U Riedel

Abstract

Bifidobacteria are an important group of the human intestinal microbiota that have been shown to exert a number of beneficial probiotic effects on the health status of their host. Due to these effects, bifidobacteria have attracted strong interest in health care and food industries for probiotic applications and several species are listed as so-called "generally recognized as safe" (GRAS) microorganisms. Moreover, recent studies have pointed out their potential as an alternative or supplementary strategy in tumor therapy or as live vaccines. In order to study the mechanisms by which these organisms exert their beneficial effects and to generate recombinant strains that can be used as drug delivery vectors or live vaccines, appropriate molecular tools are indispensable. This review provides an overview of the currently available methods and tools to generate recombinant strains of bifidobacteria. The currently used protocols for transformation of bifidobacteria, as well as replicons, selection markers, and determinants of expression, will be summarized. We will further discuss promoters, terminators, and localization signals that have been used for successful generation of expression vectors.

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Citations

Feb 6, 2014·International Journal of Food Microbiology·José M LandeteJuan L Arqués
Mar 24, 2016·Journal of Proteomics·Lorena RuizAbelardo Margolles
Mar 18, 2015·Biotechnology Letters·Cynthia Montenegro-RodríguezJosé M Landete
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Jun 6, 2016·World Journal of Microbiology & Biotechnology·José M LandeteJuan L Arqués
Jul 6, 2016·Frontiers in Microbiology·Amy O'Callaghan, Douwe van Sinderen
Mar 4, 2014·Applied and Environmental Microbiology·Verena GrimmChristian U Riedel
Jul 19, 2017·Scientific Reports·Lorena RuizDouwe van Sinderen
May 18, 2019·Proceedings of the National Academy of Sciences of the United States of America·Christopher D JohnstonKatherine P Lemon

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