Accessing the position-specific 18O/16O ratios of lignin monomeric units from higher plants with highly selective hydrogenolysis followed by GC/Py/IRMS analysis.

Analytica Chimica Acta
Ying WangYouping Zhou

Abstract

The 18O/16O of lignin at bulk, molecular and positional levels can be used to extract valuable information about climate, plant growth environment, plant physiology, and plant metabolism. Access to the individual oxygen isotope compositions (δ18O) in the lignin monomeric units is, however, challenging as depolymerization of lignin to release the monomeric units may cause isotope fractionation. We have developed a novel method to measure the δ18O of the three oxygens (O-3, O-4 and O-5) attached to the aromatic ring of the monomeric units (bearing no oxygen in their side chains) releasable by highly selective W2C/AC (tungsten carbide supported by activated carbon)-catalyzed hydrogenolysis of lignin. O-4 is obtained by measuring the δ18O of H-type monomeric unit, while O-3 and O-5 can be calculated following isotope mass balance between H, G and S-type monomeric units measurable simultaneously with GC/Py/IRMS (gas chromatography-pyrolysis-isotope ratio mass spectrometry). The measurement precisions are better than 1.15 mUr and 4.15 mUr at molecular and positional levels, respectively. It was shown that there were a δ18OH > δ18OG > δ18OS isotopic order in the herbaceous plant lignin and an (inclusive) opposite order in woody plant ...Continue Reading

References

Aug 29, 2001·Phytochemistry·H L SchmidtA Rossmann
May 18, 2010·Plant Physiology·Ruben VanholmeWout Boerjan
Feb 26, 2015·Global Change Biology·Steven J HallKenneth E Hammel
Jun 4, 2016·Nature Plants·Jaime BarrosRichard A Dixon
Apr 25, 2017·Plant, Cell & Environment·Marco M LehmannMatthias Saurer
Dec 5, 2019·Open Biology·Richard A Dixon, Jaime Barros

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