Accessory cell function of a human colonic epithelial cell line HT-29 for bacterial superantigens

Clinical and Experimental Immunology
Z X LiuT Toyota

Abstract

The expression and up-regulation of cell adhesion molecules on a human colonic epithelial cell line HT-29, and the peripheral blood T lymphocyte proliferation responses to bacterial superantigens presented by this cell line were investigated, compared with peripheral blood monocytes. In HT-29 cells, there was constitutive expression of intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-3 (LFA-3) at a low level, but no constitutive expression of HLA-DR, LFA-1, B7-1 and B7-2 molecules. After stimulation with the supernatants of staphylococcal enterotoxin B (SEB)-stimulated peripheral blood mononuclear cells for 48 h, there was significant up-regulation of HLA-DR and ICAM-1 molecules (both > 90% positive). However, this stimulation had no effect on the expression of LFA-1, B7-1, B7-2 and LFA-3 molecules. In the presence of all tested superantigens SEB, toxic shock syndrome toxin-1, and streptococcal pyogenic exotoxin A, stimulated HT-29 cells caused significant T cell proliferation. When monocytes were used as antigen-presenting cells (APC), the MoAbs against HLA-DR, B7-2 and LFA-3 showed a significant inhibition of SEB-induced T cell proliferation. Anti-ICAM-1 MoAb had no effect on this response...Continue Reading

Citations

Jul 2, 1998·Clinical and Experimental Immunology·F A ArosaD N Posnett
Nov 11, 2003·Clinical and Diagnostic Laboratory Immunology·Toshinobu KuroishiKatsuo Kumagai
May 4, 1999·Therapeutic Apheresis : Official Journal of the International Society for Apheresis and the Japanese Society for Apheresis·M NoguchiT Toyota
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Irina V PinchukVictor E Reyes

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