Accessory enzymes influence cellulase hydrolysis of the model substrate and the realistic lignocellulosic biomass

Enzyme and Microbial Technology
Fubao Fuebiol SunSong Shen

Abstract

The potential of cellulase enzymes in the developing and ongoing "biorefinery" industry has provided a great motivation to develop an efficient cellulase mixture. Recent work has shown how important the role that the so-called accessory enzymes can play in an effective enzymatic hydrolysis. In this study, three newest Novozymes Cellic CTec cellulase preparations (CTec 1/2/3) were compared to hydrolyze steam pretreated lignocellulosic substrates and model substances at an identical FPA loading. These cellulase preparations were found to display significantly different hydrolytic performances irrelevant with the FPA. And this difference was even observed on the filter paper itself when the FPA based assay was revisited. The analysis of specific enzyme activity in cellulase preparations demonstrated that different accessory enzymes were mainly responsible for the discrepancy of enzymatic hydrolysis between diversified substrates and various cellulases. Such the active role of accessory enzymes present in cellulase preparations was finally verified by supplementation with β-glucosidase, xylanase and lytic polysaccharide monooxygenases AA9. This paper provides new insights into the role of accessory enzymes, which can further provid...Continue Reading

References

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Oct 24, 2006·Biotechnology and Bioengineering·Alex BerlinJack Saddler
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Citations

Oct 20, 2015·Applied Microbiology and Biotechnology·Thamy Lívia Ribeiro CorrêaGonçalo Amarante Guimarães Pereira
Aug 26, 2016·Bioresource Technology·Hui-Ting SongZheng-Bing Jiang
Sep 11, 2016·Current Opinion in Chemical Biology·Christian P Kubicek, Eva M Kubicek
Aug 26, 2020·Journal of Industrial Microbiology & Biotechnology·Heidi ØstbyAnikó Várnai
May 31, 2017·Microbial Biotechnology·Irina S Druzhinina, Christian P Kubicek
Aug 14, 2019·Applied Microbiology and Biotechnology·Danilo Elton EvangelistaIgor Polikarpov
Jul 31, 2019·Biotechnology for Biofuels·Pooja DixitColin James Barrow
May 14, 2020·FEMS Yeast Research·Pamela Magalí BermejoAndreas Karoly Gombert
Dec 26, 2019·Enzyme and Microbial Technology·Lorena Cardoso CintraCirano José Ulhoa
Apr 30, 2017·International Journal of Biological Macromolecules·Antonielle Vieira Monclaro, Edivaldo Ximenes Ferreira Filho
Jan 15, 2017·Microbiology Spectrum·Miia R MäkeläKristiina Hildén
Aug 21, 2021·Frontiers in Bioengineering and Biotechnology·Abel T IngleDaniel R Noguera

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