Accessory gene regulator locus of Staphylococcus intermedius.

Infection and Immunity
Julia M L SungDavid H Lloyd

Abstract

The accessory gene regulator (agr) locus, a candidate system for the regulation of the production of virulence factors in Staphylococcus intermedius, has been characterized. Using PCR-based genome walking, we have obtained the first complete sequence (3,436 bp) of the accessory gene regulator (agr) gene in this organism. Sequence analysis of the agr gene has identified five open reading frames (ORFs), agrB, agrD, agrC, agrA, and hld. The translated ORF contained amino acid motifs characteristic of the response regulator and histidine protein kinase signal transducer of the classic two-component regulatory system. Sequencing of the agrD PCR products amplified from DNA from 20 different isolates has facilitated detection of genetic variation in the putative autoinducing peptide (AIP) within the agr gene of S. intermedius, revealing the presence of at least three agr specificity groups within this species. Classification of the agr gene from S. intermedius was supported by phylogenetic analysis. Real-time PCR also revealed that the effector molecule of the agr system, RNAIII, was regulated in an autocrine manner in S. intermedius and demonstrated positive correlation with the temporal gene expression patterns of luk and entC. Tran...Continue Reading

References

May 11, 1990·Science·P Marrack, J Kappler
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Mar 1, 1987·The British Veterinary Journal·P J Ihrke
Feb 7, 1982·Journal of Theoretical Biology·J H Freer, T H Birkbeck
Aug 30, 1995·Molecular & General Genetics : MGG·R P NovickS Moghazeh
May 1, 1993·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·R T Greene, C Lämmler
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·G JiR P Novick
Nov 1, 1996·Archives of Biochemistry and Biophysics·K DziewanowskaD J Guerra
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·P MayvilleT W Muir
Dec 23, 1999·Current Opinion in Microbiology·P Tortosa, D Dubnau
Aug 2, 2001·Journal of Applied Toxicology : JAT·R P ChilcottP Rice
Dec 26, 2001·The Journal of Biological Chemistry·Gholson J LyonTom W Muir
Feb 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Markus KalkumBrian T Chait
May 14, 2003·Veterinary Microbiology·Rie TerauchiNobutoshi Maehara

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Citations

Oct 31, 2006·Analytical and Bioanalytical Chemistry·Niamh HarraghyMathias Herrmann
Dec 23, 2010·Chemical Reviews·Matthew ThoendelAlexander R Horswill
Aug 19, 2007·Applied and Environmental Microbiology·Cheryl L MaloneAlexander R Horswill
Aug 21, 2008·Annual Review of Genetics·Richard P Novick, Edward Geisinger
Apr 21, 2012·Veterinary Dermatology·Jeanette Bannoehr, Luca Guardabassi
Mar 26, 2011·Microbiology and Immunology·Regina Adriana de Oliveira CalsolariMaria de Lourdes Ribeiro de Souza da Cunha
Dec 3, 2021·PLoS Pathogens·Karen C CarrollLars F Westblade

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