Jun 11, 2014

Age and neuroinflammation are important components of the mechanism of cognitive and neurobehavioral deficits in sickle cell disease.

BioRxiv : the Preprint Server for Biology
Carlo G. ArtieriHyacinth I Hyacinth


Objectives: Cognitive and neurobehavioral abnormalities are the most common and complex complications of sickle cell disease (SCD). Known risk factors influencing abnormalities are stroke and silent cerebral infarcts, but a majority of cases do not have overt cerebral injury and the underlying mechanism is not well understood. This study aims to determine whether sickle cell mice could recapitulate features of cognitive and neurobehavioral impairment observed in sickle cell patients as well as to determine the underlying cellular mechanism of these SCD complications. Methods: Using a longitudinal cross-sectional study design, we evaluated cognition and neurobehavioral deficits as an outcome. Six as well as 13 months old male Townes humanized sickle cell (SS) and matched control (AA) mice were tested. The combination of novel object recognition and fear conditioning tests was employed to measure anxiety/depression, learning and memory. Immunohistochemistry was performed to quantify bone marrow-derived microglia (CD45+) and activated microglia (Iba1+) in the dentate and peri-dentate gyrus to determine if these factors were potential pathogenic mechanisms associated with cognitive and neurobehavioral abnormalities. We evaluated ne...Continue Reading

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Mentioned in this Paper

In Vivo
Peptide Biosynthesis
Amino Acids, I.V. solution additive
Nucleic Acid Sequencing
Molecular Profiling
Profile (Lab Procedure)
Protein Biosynthesis

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