Accounting for filter bandwidth improves the quantitative accuracy of bioluminescence tomography

Journal of Biomedical Optics
Shelley L TaylorHamid Dehghani

Abstract

Bioluminescence imaging is a noninvasive technique whereby surface weighted images of luminescent probes within animals are used to characterize cell count and function. Traditionally, data are collected over the entire emission spectrum of the source using no filters and are used to evaluate cell count/function over the entire spectrum. Alternatively, multispectral data over several wavelengths can be incorporated to perform tomographic reconstruction of source location and intensity. However, bandpass filters used for multispectral data acquisition have a specific bandwidth, which is ignored in the reconstruction. In this work, ignoring the bandwidth is shown to introduce a dependence of the recovered source intensity on the bandwidth of the filters. A method of accounting for the bandwidth of filters used during multispectral data acquisition is presented and its efficacy in increasing the quantitative accuracy of bioluminescence tomography is demonstrated through simulation and experiment. It is demonstrated that while using filters with a large bandwidth can dramatically decrease the data acquisition time, if not accounted for, errors of up to 200% in quantitative accuracy are introduced in two-dimensional planar imaging, ...Continue Reading

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Citations

Jan 8, 2019·Biomedical Optics Express·Hamid DehghaniKen Kang-Hsin Wang
Dec 5, 2019·Biomedical Optics Express·Alexander BentleyHamid Dehghani

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