Accounting for the Central Role of Interfacial Water in Protein-Ligand Binding Free Energy Calculations.

Journal of Chemical Theory and Computation
Ido Y Ben-ShalomMichael K Gilson

Abstract

Rigorous binding free energy methods in drug discovery are growing in popularity because of a combination of methodological advances, improvements in computer hardware, and workflow automation. These calculations typically use molecular dynamics (MD) to sample from the Boltzmann distribution of conformational states. However, when part or all of the binding sites is inaccessible to the bulk solvent, the time needed for water molecules to equilibrate between bulk solvent and the binding site can be well beyond what is practical with standard MD. This sampling limitation is problematic in relative binding free energy calculations, which compute the reversible work of converting ligand 1 to ligand 2 within the binding site. Thus, if ligand 1 is smaller and/or more polar than ligand 2, the perturbation may allow additional water molecules to occupy a region of the binding site. However, this change in hydration may not be captured by standard MD simulations and may therefore lead to errors in the computed free energy. We recently developed a hybrid Monte Carlo/MD (MC/MD) method, which speeds up the equilibration of water between bulk solvent and buried cavities, while sampling from the intended distribution of states. Here, we repo...Continue Reading

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Citations

Jun 30, 2021·Chemical Society Reviews·Marley L SamwaysJonathan W Essex
Aug 31, 2021·Current Opinion in Structural Biology·Eleni Michael, Thomas Simonson

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