Accumulation and interaction of hypericin in low-density lipoprotein--a photophysical study

Photochemistry and Photobiology
Prasun MukherjeePavol Miskovsky

Abstract

The accumulation and interaction of hypericin with the biologically important macromolecule, low-density lipoprotein (LDL), is investigated using various steady-state and time-resolved fluorescence measurements. It is concluded that multiple hypericins can penetrate considerably deeply into the LDL molecule. Up to approximately 20 nonaggregated hypericin molecules can enter LDL; but upon increasing the hypericin concentration, the fluorescence lifetime of hypericin decreases drastically, suggesting most likely the self-quenching of aggregated hypericin. There is also evidence of energy transfer from tryptophans of the constituent protein, apoB-100, to hypericin in LDL. The results demonstrate the ability of LDL to solubilize hypericin (a known photosensitizer) in nonaggregated form, which has implications for the construction of drug delivery systems.

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Citations

Dec 1, 2010·Photochemistry and Photobiology·Luboslava BuriankovaDaniel Jancura
Mar 5, 2009·Journal of Biomedical Optics·Jun LiuThorsten Wohland
Aug 25, 2011·The Journal of Chemical Physics·Atanu Kumar DasKankan Bhattacharyya
Sep 23, 2014·International Journal of Pharmaceutics·Jaroslava JoniovaDaniel Jancura
Jan 20, 2010·International Journal of Pharmaceutics·Veronika HuntosovaFranck Sureau
Aug 23, 2016·World Journal of Gastroenterology : WJG·Marlein Miranda ConaYi-Cheng Ni
Feb 23, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Dibyendu Kumar DasKankan Bhattacharyya
Jun 26, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Veronika HuntosovaPavol Miskovsky
Dec 8, 2009·Journal of Chemical Theory and Computation·Emma S E ErikssonLeif A Eriksson
Jan 5, 2013·The Journal of Physical Chemistry. B·Alena StrejčkováGregor Bánó
Mar 8, 2011·Journal of Chemical Theory and Computation·Emma S E Eriksson, Leif A Eriksson

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